Search

  • Contáctenos
  • Orden Rápida

Invitrogen

Phospho-IRF3 (Ser396) Monoclonal Antibody (E.875.8)

Advanced Verification
2 Published Figures
8 References
View all (70) IRF3 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
Phospho-IRF3 (Ser396) Monoclonal Antibody (E.875.8)

Product Details

MA5-14947

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 8 publications 8 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

E.875.8

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pSer396 of human IRF-3
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

46 µg/mL

Amount

4.6 µg

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10987145

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Interferon regulatory factor 3; IRF-3; IRF-3a; IRF-3b; IRF-3c; IRF-3d; IRF-3e; IRF-3f; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; phospho IRF3; unnamed protein product

View more View less

Gene Aliases: C920001K05Rik; IIAE7; IRF-3; IRF3

View more View less

UniProt ID: (Human) Q14653, (Mouse) P70671

View more View less

Entrez Gene ID: (Human) 3661, (Mouse) 54131

View more View less

Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein domain specific binding identical protein binding protein homodimerization activity sequence-specific DNA binding sequence-specific double-stranded DNA binding promoter-specific chromatin binding regulatory region DNA binding transcription regulatory region DNA binding winged helix/forkhead transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter positive regulation of cytokine production immune system process cytoplasmic pattern recognition receptor signaling pathway regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter apoptotic process cellular response to DNA damage stimulus cell surface receptor signaling pathway mRNA transcription response to bacterium regulation of gene expression programmed cell death signal transduction involved in regulation of gene expression lipopolysaccharide-mediated signaling pathway positive regulation of type I interferon production response to lipopolysaccharide positive regulation of interferon-alpha production positive regulation of interferon-beta production toll-like receptor 4 signaling pathway TRIF-dependent toll-like receptor signaling pathway MDA-5 signaling pathway regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling response to exogenous dsRNA innate immune response positive regulation of innate immune response positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of inflammatory response defense response to virus type I interferon signaling pathway positive regulation of type I interferon-mediated signaling pathway cellular response to lipopolysaccharide cellular response to exogenous dsRNA macrophage apoptotic process programmed necrotic cell death cellular response to virus antiviral innate immune response cGAS/STING signaling pathway positive regulation of cytokine production involved in inflammatory response transcription, DNA-templated type I interferon biosynthetic process negative regulation of interferon-beta biosynthetic process negative regulation of defense response to virus by host positive regulation of cytokine secretion
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support