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Invitrogen
TCF7 Monoclonal Antibody (G.452.9)
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FIGURE: 1 / 5
TCF7 Antibody (MA5-14972) in ICC/IF
Immunofluorescence analysis of TCF7 was performed using 70% confluent log phase MOLT-4 and LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TCF7 Monoclonal Antibody (3D3) (Product # MA5-14972) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Pro... View More
Product Details
MA5-14972
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
G.452.9
Immunogen
Synthetic peptide corresponding to a region surrounding Leu160 of human TCF1/TCF7 protein.
Conjugate
Form
Liquid
Concentration
163 µg/mL
Amount
16.3 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with LEF1.
Target Information
TCF7 (transcriptional factor 7) is a transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ and CD8+ immature thymocytes. Isoforms for TCF7 lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. TCF7 binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. It may also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3, and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HMG-box; OTTHUMP00000159390; OTTHUMP00000159391; T cell factor-1; T-cell factor 1; T-cell specific; T-cell-factor-7; T-cell-specific transcription factor 1; TCF-7; Transcription factor 7; transcription factor 7 (T-cell specific, HMG-box); transcription factor 7, T-cell specific; unnamed protein product
Gene Aliases: AI465550; TCF-1; TCF1; TCF7
UniProt ID: (Human) P36402, (Mouse) Q00417
Entrez Gene ID: (Human) 6932, (Rat) 363595, (Mouse) 21414
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding
DNA binding
protein binding
beta-catenin binding
sequence-specific double-stranded DNA binding
core promoter binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
molecular_function
chromatin binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
transcription regulatory region DNA binding
DNA-binding transcription factor
gene-specific transcriptional regulator
immune system process
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
immune response
Wnt signaling pathway
gamma-delta T cell differentiation
regulation of gamma-delta T cell differentiation
negative regulation of transcription, DNA-templated
canonical Wnt signaling pathway
cellular response to interleukin-4
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
brain development
biological_process
neural tube development
embryonic genitalia morphogenesis
T cell receptor V(D)J recombination
regulation of cell proliferation
canonical Wnt signaling pathway involved in negative regulation of apoptotic process
alpha-beta T cell differentiation
embryonic digestive tract morphogenesis
embryonic hindgut morphogenesis
generation of neurons
transcription, DNA-templated
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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