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Invitrogen
Phospho-PERK (Thr980) Monoclonal Antibody (G.305.4)
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FIGURE: 1 / 20
Phospho-PERK (Thr980) Antibody (MA5-15033) in WB
Western blot was performed using anti-Phospho-PERK (Thr980) Monoclonal Antibody (G.305.4) (Product # MA5-15033) and a 125 kDa band corresponding to Phospho-PERK was induced in RSC96 cells upon thapsigargin and tunicamycin treatments which are known to induce ER stress. Whole cell extracts (30 µg lysate) of RSC96 (Lane1), RSC96 treated with Thapsigargin (300nM for 2hrs) (Lane 2) and RSC96 with Tunicamycin (4... View More
Product Details
MA5-15033
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
G.305.4
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding pThr980 of mouse PERK
Conjugate
Form
Liquid
Concentration
441 µg/mL
Amount
44.1 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2.7.11.1; Eukaryotic translation initiation factor 2-alpha kinase 3; OTTHUMP00000207187; OTTHUMP00000207188; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase; Protein tyrosine kinase EIF2AK3
Gene Aliases: Eif2ak3; PEK; Perk
UniProt ID: (Rat) Q9Z1Z1
Entrez Gene ID: (Rat) 29702
skeletal system development
ossification
angiogenesis
chondrocyte development
translation
protein phosphorylation
activation of cysteine-type endopeptidase activity involved in apoptotic process
ER overload response
endoplasmic reticulum organization
lactation
positive regulation of signal transduction
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
negative regulation of gene expression
regulation of translational initiation by eIF2 alpha phosphorylation
negative regulation of translation
regulation of fatty acid metabolic process
calcium-mediated signaling
bone mineralization
endoplasmic reticulum unfolded protein response
pancreas development
endocrine pancreas development
negative regulation of myelination
negative regulation of translation in response to stress
negative regulation of translational initiation in response to stress
positive regulation of protein binding
SREBP signaling pathway
response to endoplasmic reticulum stress
cellular response to glucose starvation
negative regulation of apoptotic process
fat cell differentiation
positive regulation of transcription from RNA polymerase I promoter
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
protein homooligomerization
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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