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          • Primary Antibodies ›
          • ERK1/2 Antibodies

          Invitrogen

          ERK1/ERK2 Monoclonal Antibody (E.657.8)

          Advanced Verification
          View all (81) ERK1/2 antibodies

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          Cite ERK1/ERK2 Monoclonal Antibody (E.657.8)

          Additional Information:
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          • Antibody Testing Data (2)
          • Advanced Verification (2)
          ERK1/ERK2 Antibody in Western Blot (WB)
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          ERK1/ERK2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          ERK1/ERK2 Antibody (MA5-15227) in WB

          Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), H1975 (Lane 2), HCT 116 (Lane 3), HT-29 (Lane 4), A-431 (Lane 5), A549 (Lane 6), MDA-MB-231 (Lane 7), MCF7 (Lane 8) and U-87 MG (Lane 9). The blot was probed with Mouse Anti-ERK1/ERK2 Monoclonal Antibody (Product # MA5-15227, 1:2000) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). 42, 44 kDa band corresponding to ERK1/ERK2 was obs... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody in Western Blot (WB)
          ERK1/ERK2 Antibody
          ERK1/ERK2 Antibody

          Product Details

          MA5-15227

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000
          -
          Product Specifications

          Species Reactivity

          Bovine, Hamster, Human, Mink, Mouse, Non-human primate, Pig, Rat, Zebrafish

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          E.657.8

          Immunogen

          Synthetic peptide corresponding to the sequence of p44/42 MAP kinase

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          75 µg/mL

          Purification

          Affinity chromatography

          Storage buffer

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          Contains

          <0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10980663

          Product Specific Information

          It is not recommended to aliquot this antibody.

          This antibody is not cross-reactive with either JNK/SAPK or p38 MAP kinase.

          Target Information

          ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: ERK-1; ERK-2; erk1 erk2; ERK1b; ERK2; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase-1; extracellular signal-regulated kinase-2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 1; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; pp42/MAP kinase; protein kinase; protein tyrosine kinase ERK2

          View more View less

          Gene Aliases: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; fi06b09; HS44KDAP; HUMKER1A; I79_009500; I79_018350; Mapk; MAPK1; MAPK2; MAPK3; MNK1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3; wu:fi06b09; zERK1; zERK2

          View more View less

          UniProt ID: (Human) P28482, (Bovine) P46196, (Human) P27361, (Rat) P63086, (Mouse) P63085, (Mouse) Q63844, (Rat) P21708

          View more View less

          Entrez Gene ID: (Human) 5594, (Bovine) 327672, (Pig) 100153927, (Bovine) 531391, (Human) 5595, (Pig) 445013, (Chinese hamster) 100759514, (Rat) 116590, (Mouse) 26413, (Mouse) 26417, (Rat) 50689, (Chinese hamster) 100753149, (Zebrafish) 360144, (Zebrafish) 399480

          View more View less

          Function(s)
          phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding MAP kinase kinase activity scaffold protein binding protein kinase activity protein kinase binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
          Process(es)
          MAPK cascade activation of MAPK activity MAPK import into nucleus transcription, DNA-templated protein phosphorylation apoptotic process chemotaxis response to stress cellular response to DNA damage stimulus cell cycle signal transduction chemical synaptic transmission axon guidance positive regulation of cell proliferation fibroblast growth factor receptor signaling pathway response to toxic substance positive regulation of peptidyl-threonine phosphorylation regulation of phosphatidylinositol 3-kinase signaling viral process peptidyl-serine phosphorylation peptidyl-threonine phosphorylation sensory perception of pain cytosine metabolic process platelet activation positive regulation of cell migration thyroid gland development regulation of protein stability lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance via telomerase regulation of stress-activated MAPK cascade mammary gland epithelial cell proliferation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB signaling pathway outer ear morphogenesis response to exogenous dsRNA response to estrogen negative regulation of cell differentiation positive regulation of translation positive regulation of transcription, DNA-templated thymus development T cell receptor signaling pathway B cell receptor signaling pathway regulation of sequence-specific DNA binding transcription factor activity regulation of cytoskeleton organization positive regulation of telomerase activity Bergmann glial cell differentiation long-term synaptic potentiation face development JAK-STAT cascade involved in growth hormone signaling pathway lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade response to epidermal growth factor caveolin-mediated endocytosis regulation of Golgi inheritance cellular response to granulocyte macrophage colony-stimulating factor stimulus regulation of cellular response to heat positive regulation of telomere capping regulation of early endosome to late endosome transport positive regulation of gene expression regulation of ossification regulation of cellular pH cellular response to amino acid starvation cellular response to reactive oxygen species response to nicotine stress-activated MAPK cascade cellular response to cadmium ion cellular response to tumor necrosis factor cellular response to dopamine positive regulation of protein phosphorylation transcription initiation from RNA polymerase I promoter protein complex assembly DNA damage induced protein phosphorylation phosphorylation arachidonic acid metabolic process BMP signaling pathway positive regulation of cyclase activity positive regulation of histone phosphorylation positive regulation of histone acetylation peptidyl-tyrosine autophosphorylation positive regulation of transcription from RNA polymerase II promoter cartilage development positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway cellular response to mechanical stimulus positive regulation of xenophagy negative regulation of apolipoprotein binding organ morphogenesis response to lipopolysaccharide intracellular signal transduction cellular response to organic substance cell surface receptor signaling pathway gastrulation regulation of gene expression eye development brain development central nervous system projection neuron axonogenesis chordate embryonic development
          It has to be done as per old AB suggested Products section.
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