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FIGURE: 1 / 8
Dynamin 1 Antibody (MA5-15285) in ICC/IF
Immunofluorescence of Dynamin-1 was performed using U2OS wild-type and DNM1 KO cells that were transfected with a green or a far red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min and permeabilized with 0.1% Triton X-100. Cells were stained with the Dynam... View More
Product Details
MA5-15285
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Fruit fly,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
3G4B6
Immunogen
Purified recombinant fragment of human Dynamin-1 expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15285 targets Dynamin-1 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples.
The MA5-15285 immunogen is purified recombinant fragment of human Dynamin-1 expressed in E. Coli.
MA5-15285 detects Dynamin-1 which has a predicted molecular weight of approximately 97kDa.
Target Information
Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin's involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-dynamin; D100; Dyn1; Dynamin; Dynamin BREDNM19; Dynamin I; Dynamin, brain; Dynamin-1; Dynamin1; EC 3.6.5.5; Neuronal dynamin; OTTHUMP00000215500; unnamed protein product
Gene Aliases: AI838169; DEE31; DEE31A; DEE31B; DNM; DNM1; EIEE31; Ftfl; Kiaa4093; mKIAA4093
UniProt ID: (Human) Q05193, (Mouse) P39053, (Rat) P21575
Entrez Gene ID: (Human) 1759, (Mouse) 13429, (Rat) 140694
nucleotide binding
RNA binding
GTPase activity
protein binding
GTP binding
phosphatidylinositol-4,5-bisphosphate binding
phosphatidylinositol-3,4,5-trisphosphate binding
microtubule binding
hydrolase activity
GDP binding
protein kinase binding
identical protein binding
protein homodimerization activity
protein C-terminus binding
D2 dopamine receptor binding
protein complex binding
poly(A) RNA binding
protein dimerization activity
nitric-oxide synthase binding
membrane traffic protein
endocytosis
receptor-mediated endocytosis
endosome organization
synaptic vesicle budding from presynaptic endocytic zone membrane
protein homooligomerization
protein homotetramerization
regulation of vesicle size
clathrin coat assembly involved in endocytosis
vesicle scission
G-protein coupled receptor internalization
sensory perception of sound
adult locomotory behavior
receptor internalization
protein tetramerization
synaptic transmission, GABAergic
clathrin-mediated endocytosis
toxin transport
positive regulation of synaptic vesicle recycling
metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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