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Invitrogen
DDX4 Monoclonal Antibody (2F9H5)
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FIGURE: 1 / 7
DDX4 Antibody (MA5-15565) in ICC/IF
Immunofluorescent analysis of DDX4 (green) in NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at -20°C, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a DDX4mouse monoclonal antibody (Product # MA5-15565) at a concentration of 5 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32731) at a dilution of 1:... View More
Product Details
MA5-15565
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Protozoa
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2F9H5
Immunogen
Purified recombinant fragment of human DDX4 expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15565 targets DDX4 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15565 immunogen is purified recombinant fragment of human DDX4 expressed in E. Coli.
MA5-15565 detects DDX4 which has a predicted molecular weight of approximately 76kDa.
Target Information
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX4; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 4; DEAD box polypeptide 4; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MGC111074; Mvh; mvh / m'vasa; Probable ATP-dependent RNA helicase DDX4; unnamed protein product; VASA; Vasa homolog
Gene Aliases: AV206478; DDX4; Mvh; VASA
UniProt ID: (Human) Q9NQI0, (Mouse) Q61496
Entrez Gene ID: (Human) 54514, (Mouse) 13206
male meiosis
male meiosis I
gamete generation
germ cell development
spermatogenesis
cell differentiation
sperm motility
gene silencing by RNA
piRNA metabolic process
meiotic cell cycle
transposable element silencing by piRNA-mediated heterochromatin formation
transposable element silencing by piRNA-mediated DNA methylation
multicellular organism development
RNA secondary structure unwinding
regulation of protein localization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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