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Invitrogen
USP7 Monoclonal Antibody (5F11)
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FIGURE: 1 / 4
USP7 Antibody (MA5-15585) in WB
Western blot was performed using Anti-USP7 Monoclonal Antibody (5F11)(Product # MA5-15585) and a 128kDa band corresponding to USP7 was observed across cell line and tissue tested. Whole cell extracts (30 µg lysate) of A549 (Lane 1), HT-29 (Lane 2), HeLa (Lane 3), HCT 116 (Lane 4), MCF7 (Lane 5) and tissue extract of Rat Kidney (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System... View More
Product Details
MA5-15585
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5F11
Immunogen
Purified recombinant fragment of human HAUSP expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15585 targets HAUSP in indirect ELISA, WB applications and shows reactivity with Human samples.
The MA5-15585 immunogen is purified recombinant fragment of human HAUSP expressed in E. Coli.
.
MA5-15585 detects HAUSP which has a predicted molecular weight of approximately 128kDa.
Target Information
USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chromosome 16p13.2 deletion syndrome; Deubiquitinating enzyme 7; Herpes virus-associated ubiquitin-specific protease; Herpesvirus-associated ubiquitin-specific protease; Ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific peptidase 7 (herpes virus-associated); ubiquitin specific protease 7 (herpes virus-associated); Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7; unnamed protein product; USP7; herpes virus-associated protease
Gene Aliases: C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP; TEF1; USP7
UniProt ID: (Human) Q93009
Entrez Gene ID: (Human) 7874
autophagosome assembly
DNA repair
transcription-coupled nucleotide-excision repair
DNA dealkylation involved in DNA repair
proteolysis
cellular response to DNA damage stimulus
negative regulation of autophagy
positive regulation of autophagy
protein ubiquitination
protein deubiquitination
regulation of protein stability
cellular response to nutrient levels
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
monoubiquitinated protein deubiquitination
TORC1 signaling
regulation of circadian rhythm
hypermethylation of CpG island
negative regulation of gluconeogenesis
rhythmic process
protein stabilization
regulation of sequence-specific DNA binding transcription factor activity
regulation of establishment of protein localization to telomere
symbiont-mediated disruption of host cell PML body
regulation of signal transduction by p53 class mediator
negative regulation of TORC1 signaling
regulation of telomere capping
regulation of retrograde transport, endosome to Golgi
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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