Search
Invitrogen
EGF Monoclonal Antibody (4E11)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
Product Details
MA5-15606
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4E11
Immunogen
Purified recombinant fragment of human EGF expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15606 targets EGF in indirect ELISA, IF and WB applications and shows reactivity with Human samples.
The MA5-15606 immunogen is purified recombinant fragment of human EGF expressed in E. Coli.
.
MA5-15606 detects EGF which has a predicted molecular weight of approximately 134kDa.
Target Information
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta-urogastrone; EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); unnamed protein product
Gene Aliases: AI790464; EGF; HOMG4; URG
UniProt ID: (Human) P01133, (Mouse) P01132
Entrez Gene ID: (Human) 1950, (Mouse) 13645
angiogenesis
positive regulation of endothelial cell proliferation
positive regulation of receptor internalization
epidermal growth factor receptor signaling pathway
cell proliferation
positive regulation of cell proliferation
positive regulation of endothelial cell migration
positive regulation of macromolecule metabolic process
positive regulation of gene expression
positive regulation of peptidyl-threonine phosphorylation
cerebellar granule cell precursor proliferation
positive regulation of cerebellar granule cell precursor proliferation
positive regulation of cell migration
ERBB2-EGFR signaling pathway
positive regulation of phosphorylation
positive regulation of DNA binding
positive regulation of MAPK cascade
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
regulation of JAK-STAT cascade
system development
branching morphogenesis of an epithelial tube
epithelial cell proliferation
positive regulation of epithelial cell proliferation
negative regulation of secretion
regulation of transport
positive regulation of protein kinase B signaling
mammary gland alveolus development
ERK1 and ERK2 cascade
regulation of primary metabolic process
positive regulation of canonical Wnt signaling pathway
regulation of calcium ion import
negative regulation of cholesterol efflux
positive regulation of hyaluronan biosynthetic process
positive regulation of protein localization to early endosome
positive regulation of epithelial tube formation
regulation of protein localization to cell surface
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
activation of MAPKK activity
STAT protein import into nucleus
peptidyl-tyrosine phosphorylation
positive regulation of catenin import into nucleus
positive regulation of MAP kinase activity
positive regulation of epidermal growth factor-activated receptor activity
regulation of protein secretion
regulation of peptidyl-tyrosine phosphorylation
regulation of protein transport
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support