Invitrogen

FAK Monoclonal Antibody (10H7A6)

Name: FAK Monoclonal Antibody (10H7A6)
Brand: Invitrogen
SKU: MA5-15643

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1 Published Figure

1 Reference

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FIGURE: 1 / 3

FAK Antibody (MA5-15643) in WB

Western blot analysis of FAK using FAK monoclonal antibody (Product # MA5-15643) in FAK (AA: 354-533) human IgG Fc transfected HEK293 cell lysate.

Product Image Gallery

FAK Antibody — Fig. 4. PKA subunit RIIbeta agonist inhibits TGFbeta-1-stimulated phosphorylation of FAK and relative expression of SRF. A and B: reduced level of phosphorylation of FAK by PKARIIbeta agonist (Sp-5,6-DCI-cBIMPs) (B) but not PKARIbeta agonist (2-Cl-8-MA-cAMP) (A). After 24 h of serum starvation, cells were treated with PKAR specific agonists (500 muM) for 30 min before the addition of TGFbeta-1 (5 ng/mL), and cells were harvested 24 h thereafter for Western blot analysis. Blots are from one representative experiment, and graph shows mean and SE values from densitometric analysis of n = 4 independent experiments for p-FAK. *Significant difference from TGFbeta-1 alone, P < 0.001. C and D: inhibition of SRF mRNA (C) and protein (D) expression by PKARIIbeta agonist. After 48 h of serum starvation, cells were treated with PKAR specific agonists (500 muM) for 30 min before the addition of TGFbeta-1 (5 ng/mL), and cells were harvested 24 h thereafter for mRNA analysis by qPCR and protein analysis by Western blot. The graph shows mean and SE values from relative expression of n = 4 independent experiments. Densitometry of SRF protein blots was normalized to GAPDH. Relative expression of SRF was normalized to GAPDH. E: effect of R isoform-specific knockdown on PGE 2 inhibition of FAK protein phosphorylation by TGFbeta-1 After incubation for 4 days with R isoform-specific siRNA, HLFs were treated for 30 min with or without PGE 2 (500 nM) before addit {TM}

Product Details

MA5-15643

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10H7A6

Immunogen

Purified recombinant fragment of human FAK expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10988858

Product Specific Information

MA5-15643 targets FAK in indirect ELISA, WB applications and shows reactivity with Human samples.

The MA5-15643 immunogen is purified recombinant fragment of human FAK expressed in E. Coli.
.

MA5-15643 detects FAK which has a predicted molecular weight of approximately 119kDa.

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK-Del33; exon 33 deletion splicing mutant; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; unnamed protein product

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Gene Aliases: FADK; FADK 1; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71; PTK2

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UniProt ID: (Human) Q05397

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Entrez Gene ID: (Human) 5747

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Function(s)

nucleotide binding actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein tyrosine phosphatase activity integrin binding protein binding ATP binding JUN kinase binding kinase activity transferase activity protein kinase binding protein phosphatase binding SH2 domain binding molecular function activator activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FAK Monoclonal Antibody (10H7A6)

FAK Antibody (MA5-15643) in WB

Product Details

MA5-15643

Western Blot (WB)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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