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Invitrogen
DOG-1 Recombinant Rabbit Monoclonal Antibody (SP31)
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FIGURE: 1 / 8
DOG-1 Antibody (MA5-16358) in ICC/IF
Immunofluorescence analysis of DOG-1 was performed using 70% confluent log phase PC-3 and DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. PC-3 cells were labeled with DOG-1 Monoclonal Antibody (Product # MA5-16358) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More
Product Details
MA5-16358
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
proprietary
Class
Recombinant Monoclonal
Type
Antibody
Clone
SP31
Immunogen
Synthetic peptides of human DOG-1 protein (aa 400-500).
Conjugate
Form
Liquid
Concentration
0.057 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is gastrointestinal stromal tumor.
Target Information
DOG-1, the novel marker, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of kit or PDGFR alpha mutation status.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ano1; anoctamin 1, calcium activated chloride channel; Anoctamin-1; Ca2+-activated Cl- channel; cacium activated chloride channel; calcium activated chloride channel; Discovered on gastrointestinal stromal tumors protein 1; FLJ10261; membrane protein; oral cancer overexpressed 2; Oral cancer overexpressed protein 2; Transmembrane protein 16A; transmembrane protein 16A (eight membrane-spanning domains); Tumor-amplified and overexpressed sequence 2; unnamed protein product
Gene Aliases: ANO1; DOG1; INDMS; MYMY7; ORAOV2; TAOS2; TMEM16A
UniProt ID: (Human) Q5XXA6
Entrez Gene ID: (Human) 55107
receptor binding
calcium activated cation channel activity
intracellular calcium activated chloride channel activity
voltage-gated chloride channel activity
chloride channel activity
protein binding
iodide transmembrane transporter activity
identical protein binding
protein homodimerization activity
metal ion binding
protein dimerization activity
ion transport
chloride transport
phospholipase C-activating G-protein coupled receptor signaling pathway
iodide transport
ion transmembrane transport
cellular response to heat
detection of temperature stimulus involved in sensory perception of pain
mucus secretion
protein localization to membrane
cation transmembrane transport
glial cell projection elongation
cellular response to peptide
chloride transmembrane transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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