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Invitrogen
Ku80 Monoclonal Antibody (SP130)
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FIGURE: 1 / 6
Ku80 Antibody (MA5-16375) in ICC/IF
Immunofluorescence analysis of Ku80 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Ku80 Monoclonal Antibody (Product # MA5-16375) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27... View More
Product Details
MA5-16375
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP130
Immunogen
Synthetic peptide corresponding to an internal region of human Ku80 protein.
Conjugate
Form
Liquid
Concentration
0.006 mg/mL
Amount
3 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.
Target Information
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein Ku80; DNA repair protein XRCC5; FLJ39089; HGNC:12833; Ku (p70/p80) subunit; Ku antigen; Ku autoantigen, 80kDa; Ku-80; Ku-86; Ku80; Ku86; Ku86 autoantigen related protein 1; Lupu; lupus; Lupus Ku autoantigen protein p86; Nuclear factor IV; OTTHUMP00000164061; OTTHUMP00000206791; Thyroid-lupus autoantigen; TLAA; unnamed protein product; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair cross-complementing protein 5
Gene Aliases: G22P2; KARP-1; KARP1; KU80; Ku86; KUB2; NFIV; XRCC5
UniProt ID: (Human) P13010
Entrez Gene ID: (Human) 7520
nucleotide binding
transcription regulatory region sequence-specific DNA binding
DNA binding
DNA helicase activity
damaged DNA binding
double-stranded DNA binding
double-stranded telomeric DNA binding
RNA binding
helicase activity
protein binding
ATP binding
enzyme activator activity
DNA-dependent ATPase activity
hydrolase activity
ATPase activity
ubiquitin protein ligase binding
U3 snoRNA binding
telomeric DNA binding
macromolecular complex binding
DNA end binding
5'-deoxyribose-5-phosphate lyase activity
telomere maintenance
recombinational repair
activation of innate immune response
immune system process
DNA repair
double-strand break repair
double-strand break repair via nonhomologous end joining
DNA recombination
cellular response to DNA damage stimulus
telomere maintenance via telomerase
negative regulation of macromolecule biosynthetic process
neurogenesis
regulation of telomere maintenance
small-subunit processome assembly
ribosome biogenesis
innate immune response
positive regulation of protein kinase activity
negative regulation of transcription, DNA-templated
regulation of smooth muscle cell proliferation
positive regulation of neurogenesis
hematopoietic stem cell differentiation
protein localization to chromosome, telomeric region
hematopoietic stem cell proliferation
cellular response to gamma radiation
negative regulation of t-circle formation
cellular response to leukemia inhibitory factor
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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