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Invitrogen
iNOS Monoclonal Antibody (SP126)
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FIGURE: 1 / 2
iNOS Antibody (MA5-16422) in IHC (P)
Immunohistochemical (paraffin) analysis of Nitric Oxide Synthase-Inducible using anti-Nitric Oxide Synthase-Inducible Monoclonal Antibody (Product # MA5-16422) in Lung Cancer Tissue. The recommended dilution for this antibody in immunohistochemistry applications is 1:100.
Product Details
MA5-16422
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP126
Immunogen
Synthetic peptide near the C-terminus of mouse iNOS protein.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Purification
Protein A/G
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is lung tissue.
Target Information
iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: calcium-calmodulin independent nitric oxide synthase iNOS; HEP-NOS; Hepatocyte NOS; hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS type II; NOS, type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2; unnamed protein product
Gene Aliases: HEP-NOS; i-NOS; INOS; Inosl; NOS; Nos-2; NOS-II; NOS2; NOS2A
UniProt ID: (Human) P35228, (Mouse) P29477
Entrez Gene ID: (Human) 4843, (Mouse) 18126
nitric-oxide synthase activity
protein binding
calmodulin binding
FMN binding
oxidoreductase activity
heme binding
tetrahydrobiopterin binding
arginine binding
protein homodimerization activity
metal ion binding
flavin adenine dinucleotide binding
NADP binding
actin binding
NADPH-hemoprotein reductase activity
receptor binding
iron ion binding
beta-catenin binding
cAMP-dependent protein kinase regulator activity
protein kinase binding
cadherin binding
nitric-oxide synthase binding
Hsp90 protein binding
oxidoreductase
response to hypoxia
positive regulation of leukocyte mediated cytotoxicity
innate immune response in mucosa
arginine catabolic process
superoxide metabolic process
nitric oxide biosynthetic process
inflammatory response
nitric oxide mediated signal transduction
circadian rhythm
response to bacterium
response to hormone
negative regulation of gene expression
peptidyl-cysteine S-nitrosylation
prostaglandin secretion
response to lipopolysaccharide
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
Fc-gamma receptor signaling pathway involved in phagocytosis
regulation of cell proliferation
negative regulation of protein catabolic process
defense response to bacterium
regulation of cellular respiration
cell redox homeostasis
negative regulation of blood pressure
regulation of insulin secretion
defense response to Gram-negative bacterium
cellular response to lipopolysaccharide
cellular response to interferon-gamma
cellular response to xenobiotic stimulus
regulation of cytokine production involved in inflammatory response
ovulation from ovarian follicle
positive regulation of guanylate cyclase activity
cellular response to drug
regulation of protein kinase activity
positive regulation of vasodilation
positive regulation of killing of cells of other organism
oxidation-reduction process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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