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Invitrogen
Calprotectin Monoclonal Antibody (MAC387)
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FIGURE: 1 / 5
Calprotectin Antibody (MA5-16808) in ICC/IF
Immunofluorescence analysis of Calprotectin was performed using log phase THP-1 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Calprotectin Monoclonal Antibody (MAC387) (Product # MA5-16808) at 1:250 dilution in 0.1% BSA, incubated at 4 degr... View More
Product Details
MA5-16808
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Deer,
Horse,
Cat,
Guinea pig,
Goat,
Human,
Non-human primate,
Pig,
Rabbit,
Rat
Published species
Dog,
Human,
Non-human primate,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MAC387
Immunogen
Human monocytes
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is human spleen. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01EU/ug.
Mouse anti Human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain.
Target Information
The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AI323541; B8Ag; BEE11; BEE22; calcium binding protein; S100 protein; calgranulin A; calgranulin B; Calgranulin-A; Calgranulin-B; calprotectin; Calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; Cystic fibrosis antigen; intracellular calcium-binding protein (MRP14); Leukocyte L1 complex heavy chain; Leukocyte L1 complex light chain; macrophage migration inhibitory factor-related protein-14; macrophage migration inhibitory factor-related protein-8; Migration inhibitory factor-related protein 14; Migration inhibitory factor-related protein 8; MRP-14; MRP-8; Myeloid-related protein 14; Neutrophil cytosolic 23 kDa protein; Neutrophil cytosolic 7 kDa protein; p14; p23; P7; p8; Protein S100-A8; Protein S100-A9; S100 calcium binding protein A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100A8; S100A8/S100A9; S100A9; unnamed protein product; Urinary stone protein band A
Gene Aliases: 60B8AG; CAGA; CAGB; CFAG; CGLA; CGLB; CP-10; L1AG; LIAG; MA387; MAC387; MIF; MRP-14; MRP-8; MRP14; MRP8; NIF; P14; P8; S100-A8; S100-A9; S100A8; S100A9
UniProt ID: (Human) P05109, (Bovine) P28782, (Bovine) P28783, (Human) P06702, (Rabbit) P50117, (Rat) P50115, (Rat) P50116
Entrez Gene ID: (Guinea pig) 100735514, (Horse) 100061766, (Dog) 490461, (Human) 6279, (Bovine) 616818, (Pig) 100127488, (Rabbit) 100008985, (Goat) 102168876, (Dog) 490463, (Bovine) 532569, (Pig) 100127489, (Human) 6280, (Horse) 100061730, (Rabbit) 100008704, (Goat) 102182273, (Rat) 116547, (Guinea pig) 100735238, (Rat) 94195
immune system process
leukocyte migration involved in inflammatory response
chronic inflammatory response
peptide secretion
positive regulation of peptide secretion
autophagy
apoptotic process
chemotaxis
inflammatory response
response to zinc ion
astrocyte development
peptidyl-cysteine S-nitrosylation
positive regulation of cell growth
neutrophil chemotaxis
response to lipopolysaccharide
regulation of toll-like receptor signaling pathway
autocrine signaling
defense response to bacterium
endothelial cell migration
innate immune response
response to ethanol
positive regulation of inflammatory response
defense response to fungus
regulation of cytoskeleton organization
neutrophil aggregation
positive regulation of intrinsic apoptotic signaling pathway
activation of cysteine-type endopeptidase activity involved in apoptotic process
cellular oxidant detoxification
cell-cell signaling
positive regulation of neuron projection development
peptidyl-cysteine S-trans-nitrosylation
regulation of respiratory burst involved in inflammatory response
antimicrobial humoral immune response mediated by antimicrobial peptide
acute inflammatory response
wound healing
leukocyte chemotaxis
actin cytoskeleton reorganization
regulation of integrin biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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