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Invitrogen
ICAM-3 Monoclonal Antibody (MEM-171), Alexa Fluor™ 488
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FIGURE: 1 / 2
ICAM-3 Antibody (MA5-18145) in Flow
Separation of human neutrophil granulocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD50 (MEM-171) Alexa Fluor® 488 Monoclonal antibody (Product # MA5-18145) using a dilution of 4 µL reagent/100 µL of peripheral whole blood.
Product Details
MA5-18145
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MEM-171
Immunogen
Human granulocytes
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes an extracellular epitope in the D2 domain of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes.
Target Information
CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD50; CDw50; ICAM-3; ICAM-R; intercellular adhesion molecule; Intercellular adhesion molecule 3; unnamed protein product
Gene Aliases: CD50; CDW50; ICAM-R; ICAM3
UniProt ID: (Human) P32942
Entrez Gene ID: (Human) 3385
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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