Invitrogen

KLF10 Monoclonal Antibody (3E11)

Name: KLF10 Monoclonal Antibody (3E11)
Brand: Invitrogen
SKU: MA5-20125

Advanced Verification

5 Published Figures

1 Reference

View all (34) KLF10 antibodies

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Additional Information:

KLF10 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

KLF10 Antibody (MA5-20125) in ICC/IF

Immunofluorescence analysis of HeLa cells using an anti-KLF10 monoclonal antibody (Product # MA5-20125). (antibody concentration 10 µg/mL).

Product Image Gallery

KLF10 Antibody — Figure 6KDM6A and KLF10 can build a positive feedback loop that is critically involved in podocyte dysfunction AQuantitative RT-PCR evaluation of KLF10 mRNA expression in kidney samples of wild-type and KLF10-knockout (KO) mice. **P < 0.0001, significant difference versus wild-type controls (Wilcoxon two-sample test; n = 8). BWestern blot analysis of KLF10 expression in kidney samples of wild-type or KLF10-KO mice treated with or without STZ at 8 weeks after the onset of diabetes. Presented experiments were performed at least three times independently. C Changes in body weight and blood glucose levels in wild-type or KLF10-KO mice with or without STZ treatment. No significant differences in body weights or blood glucose levels between diabetic wild-type mice and diabetic KLF10-KO mice were detected (Wilcoxon two-sample test; n = 8). D Levels of urinary protein excretion, weights of kidney, and levels of HbA1c in wild-type or KLF10-KO mice with or without STZ treatment. The mean relative kidney weight (%) shown in the study is determined as the percent of kidneys out of total body weight, and the HbA1c level is defined as the ratio of HbA1c to the total hemoglobin (% HbA1c; DCCT unit). *P < 0.05 versus untreated wild-type controls, # P < 0.05 versus STZ-treated wild-type mice (parametric ANOVA and a Bonferroni post hoc test; n = 8). E Immunofluorescence images of kidney sections stained with KLF10 and nephrin in wild-type or KLF10-KO mice untreated or treated with STZ. Scale b {KO}

Immunogen image

Immunogen Image KLF10 Monoclonal Antibody (3E11)

Product Details

MA5-20125

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

10 µg/mL

ELISA (ELISA)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

3E11

Immunogen

KLF10 (AAH11538.1, 111 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2607968

Product Specific Information

Peptide Sequence: LSDTAKPHIA APFKEEEKSP VSAPKLPKAQ ATSVIRHTAD AQLCNHQTCP MKAASILNYQ NNSFRRRTHL NVEAARKNIP CAAVSPNRSK CERNTVADVD

Target Information

KLF10 is a nuclear protein belonging to the Sp1 C2H2-type zinc-finger protein family with three C2H2-type zinc fingers. KLF10 is a transcriptional repressor involved in the regulation of cell growth and inhibits cell growth. It binds to the consensus sequence 5'-GGTGTG-3' and is induced by TGF-beta thus playing a role in linking TGF-beta-mediated signaling cascades to the regulation of pancreatic epithelial cell growth. KLF10 is involved in inducing and promoting apoptosis through the mitochondrial apoptotic pathway and may inhibit Smad7 expression in bronchial epithelial cells. Ubiquitous expression of KLF10 is seen in most of the tissues. Reports suggest that it may mediate up-regulation of TGFBI in VHL-deficient tumors and other cancers. Over expression of KLF10 may be associated with renal clear cell carcinoma and other tumors. KLF11 is a zinc finger transcription factor that binds to SP1-like sequences in epsilon- and gamma-globin gene promoters. This binding inhibits cell growth and causes apoptosis. Defects in this gene are a cause of maturity-onset diabetes of the young type 7 (MODY7). Three transcript variants encoding two different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: early growth response gene alpha; early growth response-alpha; EGR-alpha; Krueppel-like factor 10; Kruppel like factor 10; TGFB-inducible early growth response protein 1; Transforming growth factor-beta-inducible early growth response protein 1; unnamed protein product; zinc finger transcription factor TIEG

View more View less

Gene Aliases: EGR-alpha; EGRA; KLF10; TIEG; TIEG1

View more View less

UniProt ID: (Human) Q13118

View more View less

Entrez Gene ID: (Human) 7071

View more View less

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding metal ion binding sequence-specific double-stranded DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cell-cell signaling circadian rhythm negative regulation of cell proliferation cellular response to starvation bone mineralization somatic stem cell population maintenance regulation of circadian rhythm positive regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

KLF10 Monoclonal Antibody (3E11)

KLF10 Antibody (MA5-20125) in ICC/IF

Product Details

MA5-20125

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!