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Invitrogen
Phospho-RNA pol II CTD (Ser2) Monoclonal Antibody
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FIGURE: 1 / 7
Phospho-RNA pol II CTD (Ser2) Antibody (MA5-23510) in ChIP-seq
ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using 1 µg RNA Polymerase II S2p monoclonal antibody (Product # MA5-23510). The IP'd DNA was subsequently analyzed on a Genome Analyzer. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence and a 150 kb region of the X-chromosome (figure 2A and B, respect... View More
Product Details
MA5-23510
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Fruit fly,
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Immunogen
Human YSPTSPS repeat in the B1 subunit of RNA polymerase II, phosphorylated at Ser2 of the repeat sequence.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.05% sodium azide
Storage conditions
-20°C or -80°C if preferred
RRID
Target Information
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 3'-5' exoribonuclease; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II; RNA polymerase II C-terminal domain; RNA polymerase II carboxy-terminal domain; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB220; RPO21; SUA8; unnamed protein product
Gene Aliases: hRPB220; hsRPB1; NEDHIB; POLR2; POLR2A; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2
UniProt ID: (Human) P24928
Entrez Gene ID: (Human) 5430
magnesium ion binding
nucleic acid binding
DNA binding
RNA binding
DNA-directed RNA polymerase activity
RNA-directed RNA polymerase activity
protein binding
zinc ion binding
transferase activity
nucleotidyltransferase activity
hydrolase activity
kinase binding
ubiquitin protein ligase binding
RNA polymerase activity
metal ion binding
microfibril binding
promoter-specific chromatin binding
transcription, RNA-templated
transcription, DNA-templated
DNA-templated transcription, termination
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
positive regulation of gene expression
positive regulation of RNA splicing
mRNA transcription from RNA polymerase II promoter
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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