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Invitrogen
Nidogen Monoclonal Antibody (302117)
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FIGURE: 1 / 2
Nidogen Antibody (MA5-23911) in ICC/IF
Immunocytochemistry analysis of Nidogen in immersion fixed human mesenchymal stem cells differentiated into chondrocytes. Samples were incubated in Nidogen monoclonal antibody (Product # MA5-23911) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).
Product Details
MA5-23911
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
302117
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nidogen-1/Entactin Leu29-Lys1114 (Gln1113Arg)
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs and Western blots, no cross-reactivity with recombinant human Nidogen-2 is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: enactin; Entactin; epididymis secretory sperm binding protein; NID 1; NID-1; Nidogen (enactin); Nidogen-1; Nidogen-1;; unnamed protein product
Gene Aliases: NID; NID1
UniProt ID: (Human) P14543
Entrez Gene ID: (Human) 4811
cell adhesion
cell-matrix adhesion
positive regulation of cell-substrate adhesion
extracellular matrix organization
glomerular basement membrane development
positive regulation of cell adhesion
positive regulation of muscle cell differentiation
basement membrane organization
regulation of basement membrane organization
positive regulation of integrin-mediated signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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