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Invitrogen
Calcineurin B Monoclonal Antibody (212306)
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FIGURE: 1 / 2
Calcineurin B Antibody (MA5-23933) in WB
Western blot analysis of Calcineurin B in MCF‚7 human breast cancer cell line, TS1 mouse helper T cell line, and PC‚12 rat adrenal pheochromocytoma cell line. Samples were incubated in Calcineurin B monoclonal antibody (Product # MA5-23933) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti‚Mouse IgG secondary antibody. A specific band was detected for Calcineurin B at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
MA5-23933
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
212306
Immunogen
E. coli-derived recombinant human Calcineurin B Met1-Val170
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous human, mouse, and rat Calcineurin B.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin (also referred to as protein phosphatase 2B) is composed of two subunits; calcineurin A (CnA), a 60 kDa catalytic subunit which is highly homologous to protein phosphatases 1 and 2A; and a 19 kDa regulatory subunit, calcineurin B (CnB) which, like calmodulin, contains four EF-hand calcium binding sites. Calcineurin-mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. The immunosuppressive drugs, cyclosporin A and FK506, when bound to the immunophilins, cyclophilin and FKBP respectively, inhibit dephosphorylation and activation of NF-AT by calcineurin. This inhibition is caused by the immunosuppressant/immunophilin complex simultaneously binding to both CnA and CnB which does not inhibit the phosphatase activity of CnA, but rather blocks access of the substrate to the catalytic domain of CnA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ALNB1; calcineurin B, type I (19kDa); Calcineurin subunit B type 1; PP2B beta 1; PPP3R1; protein phospatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I); protein phospatase 3, regulatory subunit B, alpha isoform,type 1; Protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 regulatory subunit B alpha; Protein phosphatase 3 regulatory subunit B alpha isoform 1; protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I); unnamed protein product
Gene Aliases: CALNB1; CaNB1; CNA2; CNB; CNB1; MCIP1; PPP3R1
UniProt ID: (Human) P63098, (Mouse) Q63810, (Rat) P63100
Entrez Gene ID: (Human) 5534, (Mouse) 19058, (Rat) 29748
phosphoprotein phosphatase activity
calcium-dependent protein serine/threonine phosphatase activity
calcium ion binding
protein binding
calmodulin binding
calcium-dependent protein serine/threonine phosphatase regulator activity
phosphatase binding
protein domain specific binding
metal ion binding
enzyme binding
patterning of blood vessels
epithelial to mesenchymal transition
protein import into nucleus
heart development
Schwann cell development
myelination in peripheral nervous system
calcineurin-NFAT signaling cascade
protein localization to nucleus
positive regulation of transcription from RNA polymerase II promoter
lung epithelial cell differentiation
positive regulation of calcineurin-NFAT signaling cascade
regulation of synaptic vesicle cycle
regulation of postsynaptic neurotransmitter receptor internalization
postsynaptic modulation of chemical synaptic transmission
positive regulation of calcium ion import across plasma membrane
negative regulation of calcium ion import across plasma membrane
protein dephosphorylation
NFAT protein import into nucleus
positive regulation of NFAT protein import into nucleus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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