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Invitrogen
CD300f Monoclonal Antibody (234903)
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FIGURE: 1 / 1
CD300f Antibody (MA5-23943) in ICC/IF
Immunocytochemistry analysis of CD300f was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs). The cells were labeled with CD300f Rat Monoclonal Antibody (Product # MA5-23943) using a dilution of 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) counterstained with DAPI (blue). Specific staining was localized to cell surfaces.
Product Details
MA5-23943
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
234903
Immunogen
Y3 rat myeloid cell line transfected with human CD300f/LMIR3
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, no cross-reactivity with recombinant human LMIR1, 2, 4, 5, 6, or recombinant mouse LMIR3 is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
CLM1 is an inhibitory receptor of the Ig superfamily expressed on myeloid cells. It mediates negative regulatory signals by recruiting SHP1 (PTPN6; MIM 176883) or SHIP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD300 antigen-like family member F; CD300f; CLM-1; CMRF35-like molecule 1; IgSF13; immune receptor; Immune receptor expressed on myeloid cells 1; immunoglobin superfamily member 13; Immunoglobulin superfamily member 13; inhibitory receptor IREM1; IREM-1; IREM-1 Sv1; IREM-1 Sv2; NK inhibitory receptor; NKIR; unnamed protein product
Gene Aliases: CD300F; CD300LF; CLM-1; CLM1; IGSF13; IREM-1; IREM1; LMIR3; NKIR; UNQ3105/PRO10111
UniProt ID: (Human) Q8TDQ1
Entrez Gene ID: (Human) 146722
immune system process
immune response-activating signal transduction
negative regulation of mast cell activation
negative regulation of MyD88-dependent toll-like receptor signaling pathway
TRIF-dependent toll-like receptor signaling pathway
interleukin-13-mediated signaling pathway
positive regulation of interleukin-4-mediated signaling pathway
negative regulation of apoptotic cell clearance
positive regulation of apoptotic cell clearance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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