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Invitrogen
NAMPT Monoclonal Antibody (362616)
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FIGURE: 1 / 6
NAMPT Antibody (MA5-24108) in WB
Western blot was performed using Anti-NAMPT Monoclonal Antibody (362616)(Product # MA5-24108) and a 55 kDa band corresponding to NAMPT was observed across cell lines tested. Increased expression of NAMPT was observed in HUVEC upon IL1-beta and TNF-alpha treatment. Whole cell extracts (30 µg lysate) of HUVEC (Lane 1), HUVEC treated with IL1-beta (10 ng/mL for 18hr) (Lane 2), HUVEC treated with TNF-alpha (10 ng/mL for 18hr) (Lane 3) and U-87 MG (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Reso... View More
Product Details
MA5-24108
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
362616
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PBEF/Visfatin Met1-His491
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human PBEF is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Visfatin, also known as pre-B cell colony-enhancing factor, is an adipocytokine that is abundantly secreted by visceral adipose tissue, with insulin mimetic effects. Visfatin mimics insulin signaling by binding to the insulin receptor and reducing glucose release from liver cells, while stimulating glucose utilization in adipocytes and myocytes. Visfatin is also invoved in enhancement of cell proliferation, biosynthesis of nicotinamide mono-and dinucleotide and glucose homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp666B131; EC 2.4.2.12; MGC117256; NAmPRTase; Nicotinamide phosphoribosyltransferase; PBEF; Pre-B cell-enhancing factor; Pre-B-cell colony-enhancing factor 1; Pre-B-cell colony-enhancing factor 1 homolog; unnamed protein product; Visfatin
Gene Aliases: 1110035O14Rik; AI314458; AI480535; NAmPRTase; NAMPT; PBEF; PBEF1; VF; VISFATIN
UniProt ID: (Human) P43490, (Mouse) Q99KQ4
Entrez Gene ID: (Human) 10135, (Mouse) 59027
cytokine activity
protein binding
transferase activity
transferase activity, transferring glycosyl groups
identical protein binding
nicotinamide phosphoribosyltransferase activity
nicotinate-nucleotide diphosphorylase (carboxylating) activity
drug binding
protein homodimerization activity
transferase
NADP biosynthetic process
nicotinamide metabolic process
inflammatory response
signal transduction
cell-cell signaling
circadian rhythm
positive regulation of cell proliferation
insulin receptor signaling pathway
NAD biosynthetic process
positive regulation of gene expression
pyridine nucleotide biosynthetic process
circadian regulation of gene expression
NAD salvage
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
adipose tissue development
positive regulation of ERK1 and ERK2 cascade
positive regulation of smooth muscle cell proliferation
positive regulation of nitric-oxide synthase biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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