Search
Invitrogen
WNT4 Monoclonal Antibody (55010)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
WNT4 Antibody (MA5-24376) in ICC/IF
Immunofluorescence analysis of WNT4 was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with WNT4 (55010) Rat Monoclonal Antibody (Product # MA5-24376) at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11006) a diluti... View More
Product Details
MA5-24376
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
55010
Immunogen
E. coli-derived recombinant mouse Wnt-4 (aa 37-76 and 222-295)
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Protein Wnt-4; signal molecule; unnamed protein product; wingless-related MMTV integration site 4; wingless-type MMTV integration site family, member 4
Gene Aliases: SERKAL; UNQ426/PRO864; WNT-4; WNT4
UniProt ID: (Human) P56705, (Mouse) P22724
Entrez Gene ID: (Human) 54361, (Mouse) 22417
metanephros development
branching involved in ureteric bud morphogenesis
kidney development
mesonephros development
epithelial to mesenchymal transition
embryonic epithelial tube formation
liver development
gamete generation
sex differentiation
fibroblast growth factor receptor signaling pathway
male gonad development
female gonad development
cellular response to starvation
negative regulation of gene expression
negative regulation of steroid biosynthetic process
Wnt signaling pathway
regulation of cell-cell adhesion
cell differentiation
neuron differentiation
female sex determination
adrenal gland development
negative regulation of cell migration
positive regulation of bone mineralization
positive regulation of aldosterone biosynthetic process
positive regulation of collagen biosynthetic process
T cell differentiation in thymus
immature T cell proliferation in thymus
tube morphogenesis
non-canonical Wnt signaling pathway
negative regulation of fibroblast growth factor receptor signaling pathway
hormone metabolic process
positive regulation of MAPK cascade
cell fate commitment
negative regulation of cell differentiation
positive regulation of osteoblast differentiation
positive regulation of meiotic nuclear division
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
negative regulation of Ras protein signal transduction
oocyte development
branching morphogenesis of an epithelial tube
smooth muscle cell differentiation
positive regulation of stress fiber assembly
positive regulation of focal adhesion assembly
Sertoli cell differentiation
canonical Wnt signaling pathway
somatotropin secreting cell differentiation
thyroid-stimulating hormone-secreting cell differentiation
mesenchymal to epithelial transition
tertiary branching involved in mammary gland duct morphogenesis
kidney morphogenesis
negative regulation of wound healing
mammary gland epithelium development
positive regulation of dermatome development
paramesonephric duct development
negative regulation of testicular blood vessel morphogenesis
cellular response to transforming growth factor beta stimulus
nephron development
renal vesicle formation
renal vesicle induction
metanephric mesenchymal cell differentiation
mesonephric tubule development
metanephric tubule formation
metanephric nephron development
metanephric nephron morphogenesis
apoptotic signaling pathway
meiotic nuclear division
pericyte cell differentiation
negative regulation of male gonad development
positive regulation of cortisol biosynthetic process
negative regulation of androgen biosynthetic process
negative regulation of testosterone biosynthetic process
negative regulation of apoptotic signaling pathway
androgen biosynthetic process
signal transduction
cell-cell signaling
multicellular organism development
non-canonical Wnt signaling pathway via MAPK cascade
positive regulation of GTPase activity
anatomical structure development
establishment of protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support