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Invitrogen
KDM5B Monoclonal Antibody (CL1129)
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FIGURE: 1 / 10
KDM5B Antibody (MA5-24626) in ICC/IF
Immunofluorecent analysis of KDM5B in MCF7 cells using KDM5B Monoclonal Antibody (CL1129) (Product # MA5-24626). Staining shows specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Product Details
MA5-24626
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
CL1129
Immunogen
Recombinant protein fragment of Human KDM5B (Product #RP-94108).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: DGINSLERKL KRRLEREGLS SERWERVKKM RTPKKKKIKL SHPKDMNNFK LERERSYELV RSAETHSLPS DTSYSEQEDS E
Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 91%.
Binds to an epitope located within the peptide sequence DGINSLERKLKRRLE as determined by overlapping synthetic peptides.
Target Information
KDM5B is a histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. It does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. KDM5B demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. It acts as a transcriptional corepressor for FOXG1B and PAX9. It favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, KDM5B may act as a tumor suppressor for melanoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: [histone H3]-trimethyl-L-lysine(4) demethylase 5B; Cancer/testis antigen 31; CT31; Histone demethylase JARID1B; HRIHFB2060; jumonji, AT rich interactive domain 1B; Jumonji/ARID domain-containing protein 1B; lysine (K)-specific demethylase 5B; Lysine-specific demethylase 5B; PLU-1; protein phosphatase 1, regulatory subunit 98; putative DNA/chromatin binding motif; RBP2-H1; Retinoblastoma-binding protein 2 homolog 1; retinoblastoma-binding protein 2, homolog 1A; unnamed protein product
Gene Aliases: CT31; JARID1B; KDM5B; MRT65; PLU-1; PLU1; PPP1R98; PUT1; RBBP2H1; RBBP2H1A; RBP2-H1
UniProt ID: (Human) Q9UGL1
Entrez Gene ID: (Human) 10765
nucleic acid binding
DNA binding
transcription corepressor activity
protein binding
zinc ion binding
oxidoreductase activity
histone demethylase activity
histone demethylase activity (H3-K4 specific)
histone demethylase activity (H3-trimethyl-K4 specific)
histone binding
metal ion binding
dioxygenase activity
sequence-specific double-stranded DNA binding
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
single fertilization
post-embryonic development
positive regulation of gene expression
positive regulation of mammary gland epithelial cell proliferation
cellular response to fibroblast growth factor stimulus
negative regulation of transcription, DNA-templated
rhythmic process
branching involved in mammary gland duct morphogenesis
mammary duct terminal end bud growth
response to fungicide
uterus morphogenesis
lens fiber cell differentiation
cellular response to leukemia inhibitory factor
regulation of estradiol secretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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