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Invitrogen
ABCG8 Monoclonal Antibody (1B10A5)
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FIGURE: 1 / 3
ABCG8 Antibody (MA5-24788) in ICC/IF
Immunocytochemistry analysis of ABCG8 in HepG2 cells. Samples were incubated with ABCG8 monoclonal antibody (Product # MA5-24788) using a dilution of 2 µg/mL overnight at 4°C followed by anti-mouse DyLight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Product Details
MA5-24788
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
1B10A5
Immunogen
Full length mouse ABCG8.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. ABCG8, expressed at high levels in the liver and intestine, normally cooperates with ABCG5 to limit intestinal absorption and promote biliary excretion of sterols. The mutated form of this transporter can lead to sterol accumulation and atherosclerosis or sitosterolemia, a rare autosomal recessive disorder, characterized by hyperabsorption of sterols and the inability to excrete sterols into bile.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ABCG8; ATP-binding cassette sub-family G member 8; ATP-binding cassette, sub-family G (WHITE), member 8; MGC142217; sterolin 2; Sterolin-2; Sterolin2
Gene Aliases: 1300003C16Rik; ABCG8; AI114946; GBD4; sterolin-2; STSL; STSL1
UniProt ID: (Human) Q9H221, (Mouse) Q9DBM0
Entrez Gene ID: (Human) 64241, (Mouse) 67470
protein binding
ATP binding
ATPase activity
ATPase activity, coupled to transmembrane movement of substances
metal ion binding
protein heterodimerization activity
protein dimerization activity
intermembrane cholesterol transfer activity
ABC-type transporter activity
sterol transporter activity
cholesterol transporter activity
ATP-binding cassette (ABC) transporter
primary active transporter
transporter
lipid transport
response to nutrient
response to xenobiotic stimulus
negative regulation of intestinal phytosterol absorption
response to muscle activity
phospholipid transport
sterol transport
intestinal cholesterol absorption
response to nutrient levels
cholesterol efflux
cholesterol homeostasis
negative regulation of intestinal cholesterol absorption
transmembrane transport
sterol homeostasis
triglyceride homeostasis
transport
excretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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