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Invitrogen
RBBP5 Monoclonal Antibody (1C9)
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FIGURE: 1 / 8
RBBP5 Antibody (MA5-24873) in ICC/IF
Immunofluorescence analysis of RBBP5 was performed using SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RBBP5 Monoclonal Antibody (3H3) (Product # MA5-24873) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) for 45 minutes at ... View More
Product Details
MA5-24873
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
1C9
Immunogen
Purified recombinant human RBBP5 protein fragments expressed in E.coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RBBP 5; RBBP-5; RBQ 3; Retinoblastoma-binding protein 5; Retinoblastoma-binding protein RBQ-3; SWD1, Set1c WD40 repeat protein, homolog; unnamed protein product
Gene Aliases: 4933411J24Rik; C330016J05; RBBP5; RBQ3; SWD1
UniProt ID: (Human) Q15291, (Mouse) Q8BX09
Entrez Gene ID: (Human) 5929, (Rat) 304794, (Mouse) 213464
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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