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Invitrogen
FKBP12 Monoclonal Antibody (OTI3B3)
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FIGURE: 1 / 5
FKBP12 Antibody (MA5-24953) in ICC/IF
Immunofluorescence analysis of FKBP12 was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with FKBP12 Monoclonal Antibody (OTI3B3) (Product # MA5-24953) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product ... View More
Product Details
MA5-24953
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI3B3
Immunogen
Full length human recombinant protein of FKBP1A produced in HEK293T cell
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
FKBP12 is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 12 kDa FK506-binding protein; 12 kDa FKBP; Calstabin-1; EC 5.2.1.8; FK506 binding protein 1A, 12kDa; FK506 binding protein12; FK506-binding protein (FKBP); FK506-binding protein 1; FK506-binding protein 12; FK506-binding protein 1A; FK506-binding protein, T-cell, 12-kD; FKBP-1A; FKBP12-Exip3; FKBP1A; Immunophilin FKBP12; Peptidyl-prolyl cis-trans isomerase FKBP1A; PPIase FKBP1A; protein kinase C inhibitor 2; Rotamase
Gene Aliases: FKBP-12; FKBP-1A; FKBP1; FKBP12; FKBP1A; PKC12; PKCI2; PPIASE
UniProt ID: (Human) P62942
Entrez Gene ID: (Human) 2280
peptidyl-prolyl cis-trans isomerase activity
transforming growth factor beta receptor binding
calcium channel regulator activity
protein binding
macrolide binding
FK506 binding
isomerase activity
receptor inhibitor activity
type I transforming growth factor beta receptor binding
ion channel binding
I-SMAD binding
activin receptor binding
heart morphogenesis
protein folding
'de novo' protein folding
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion
negative regulation of transforming growth factor beta receptor signaling pathway
regulation of protein localization
negative regulation of activin receptor signaling pathway
protein refolding
T cell activation
positive regulation of I-kappaB kinase/NF-kappaB signaling
regulation of immune response
protein maturation
ventricular cardiac muscle tissue morphogenesis
heart trabecula formation
fibril organization
regulation of amyloid precursor protein catabolic process
amyloid fibril formation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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