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Invitrogen
LMAN1 Monoclonal Antibody (OTI1A8)
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FIGURE: 1 / 15
LMAN1 Antibody (MA5-25345) in ICC/IF
Immunofluorescence analysis of LMAN1 was performed using 70% confluent log phase HeLa cells treated with Brefeldin A (5 µg/mL, 15 minutes). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with LMAN1 Monoclonal Antibody (OTI1A8) (Product # MA5-25345) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Seco... View More
Product Details
MA5-25345
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Non-human primate,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1A8
Immunogen
Full length human recombinant protein of LMAN1 produced in HEK293T cell
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: endoplasmic reticulum-golgi intermediate compartment protein 53; ER-Golgi intermediate compartment 53 kDa protein; ERGIC53; Gp58; Intracellular mannose-specific lectin MR60; Lectin mannose-binding 1; p58; Protein ERGIC-53; unnamed protein product
Gene Aliases: ERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; LMAN1; MCFD1; MR60; p58
UniProt ID: (Human) P49257, (Rat) Q62902
Entrez Gene ID: (Dog) 476186, (Human) 3998, (Rat) 116666
in utero embryonic development
protein folding
ER to Golgi vesicle-mediated transport
endoplasmic reticulum organization
Golgi organization
blood coagulation
gene expression
positive regulation of organelle organization
protein transport
vesicle-mediated transport
protein exit from endoplasmic reticulum
early endosome to Golgi transport
negative regulation of protein targeting to mitochondrion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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