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Invitrogen
N-WASP Monoclonal Antibody (OTI2H5)
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FIGURE: 1 / 6
N-WASP Antibody (MA5-27438) in IHC (P)
Immunohistochemistry was performed on paraffin-embedded human spleen tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a WASL monoclonal antibody (Product # MA5-27438) at a dilution of 1:150.
Product Details
MA5-27438
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2H5
Immunogen
Full length human recombinant protein of WASL produced in HEK293T cell
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Actin nucleation-promoting factor WASL; DKFZp779G0847; MGC48327; n wasp; N-WASP; Neural Wiskott-Aldrich syndrome protein; unnamed protein product; Wiskott-Aldrich syndrome like
Gene Aliases: N-WASP; NWASP; WASL; WASPB
UniProt ID: (Human) O00401
Entrez Gene ID: (Human) 8976
membrane budding
actin filament organization
actin polymerization or depolymerization
response to bacterium
vesicle organization
actin cytoskeleton organization
actin filament polymerization
vesicle transport along actin filament
protein complex localization
regulation of protein localization
positive regulation of transcription from RNA polymerase II promoter
cell division
positive regulation of filopodium assembly
spindle localization
dendritic spine morphogenesis
protein-containing complex assembly
regulation of postsynapse organization
negative regulation of membrane tubulation
positive regulation of clathrin-mediated endocytosis
negative regulation of lymphocyte migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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