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Invitrogen
CD45 Monoclonal Antibody (L12/201)
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FIGURE: 1 / 2
CD45 Antibody (MA5-28392) in Flow
Flow cytometry of CD45 in conjunctival tissue. Samples were incubated with CD45 monoclonal antibody (Product # MA5-28392). Image details are as follows: A) PBS- and LCEm- instilled groups had only a basal level of expression. CD45-positive cells (green) after instillations of BAC+latanoprost. B) were viewed after propidium iodide staining (red) and cytospin centrifugation. The scale bar indicates 50 μm. Image referenced from: Mol Vis. 2009 Aug 25;15: 1690-9.
Product Details
MA5-28392
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rabbit
Published species
Rabbit
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
L12/201
Immunogen
Glycoproteins isolated from the T cell line, RL-5
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Mouse anti Rabbit CD45 antibody, clone L12/201 recognizes the CD45 antigen, also known as leukocyte common antigen (LCA) or T200.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: T220 and B220
Entrez Gene ID: (Rabbit) 100358807
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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