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Invitrogen

CD254 (RANK Ligand) Recombinant Rabbit Monoclonal Antibody (101)

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CD254 (RANK Ligand) Recombinant Rabbit Monoclonal Antibody (101)

Product Details

MA5-29615

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

ELISA (ELISA)

1:5,000-1:50,000
-

Immunoprecipitation (IP)

1:500
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

101

Immunogen

Recombinant Human HNCH RANKL protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. not determined

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2785447

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Human RANKL/OPGL/TNFSF11/CD254.

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD254; H-RANK-L; M-Rank-L; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; rank-l; RANKL; receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TNF-related ligand; TRANCE; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; Tumor necrosis factor ligand superfamily member 11; tumor necrosis factor superfamily member 11

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Gene Aliases: CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TNFSF11; TNLG6B; TRANCE

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UniProt ID: (Human) O14788

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Entrez Gene ID: (Human) 8600

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Function(s)
receptor binding cytokine activity tumor necrosis factor receptor binding protein binding tumor necrosis factor receptor superfamily binding identical protein binding receptor agonist activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter ossification osteoclast proliferation monocyte chemotaxis immune response cell communication cell surface receptor signaling pathway JNK cascade organ morphogenesis positive regulation of gene expression cytokine-mediated signaling pathway calcium-mediated signaling signaling cell differentiation osteoclast differentiation tumor necrosis factor-mediated signaling pathway mammary gland epithelial cell proliferation positive regulation of homotypic cell-cell adhesion osteoclast development paracrine signaling positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade protein kinase B signaling tooth eruption bone resorption regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of bone resorption positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of T cell activation positive regulation of corticotropin-releasing hormone secretion positive regulation of protein kinase B signaling calcium ion homeostasis bone development mammary gland alveolus development positive regulation of ERK1 and ERK2 cascade positive regulation of fever generation by positive regulation of prostaglandin secretion positive regulation of NIK/NF-kappaB signaling positive regulation of intracellular signal transduction cellular response to leukemia inhibitory factor positive regulation of osteoclast development positive regulation of extrinsic apoptotic signaling pathway
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