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Invitrogen
STAG1 Monoclonal Antibody (GT1515)
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FIGURE: 1 / 8
STAG1 Antibody (MA5-31524) in ICC/IF
Immunofluorescence analysis of Cohesin subunit SA-1 was performed using 70% confluent log phase MCF 10A cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with STAG1 Monoclonal Antibody (GT1515) (Product # MA5-31524) at 1:500 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (P... View More
Product Details
MA5-31524
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
GT1515
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SA1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.9 mg/mL
Amount
90 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%).
Positive Control: 293T, A431, HeLa, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cohesin subunit SA-1; DKFZp781D1416; nuclear protein stromal antigen 1; SCC3 homolog 1; sister chromatid cohesion 3 homolog A; Stromal antigen 1; unnamed protein product
Gene Aliases: AU045003; MRD47; SA-1; SA1; SCC3; SCC3A; STAG1
UniProt ID: (Human) Q8WVM7, (Mouse) Q9D3E6
Entrez Gene ID: (Human) 10274, (Mouse) 20842, (Rat) 315958
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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