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Invitrogen
DKK1 Recombinant Rabbit Monoclonal Antibody (SC06-86)
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Product Details
MA5-32229
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SC06-86
Immunogen
Synthetic peptide within Human DKK1 aa 225-266.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play important regulatory roles in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: dickkopf 1 homolog; dickkopf homolog 1; Dickkopf-1; dickkopf-1 like; dickkopf-like protein 1; Dickkopf-related protein 1; Dickkopf1; hDkk1; secreted wnt inhibitor with affinity for heparin sepharose; SK; unnamed protein product
Gene Aliases: DKK-1; DKK1; SK; UNQ492/PRO1008
UniProt ID: (Human) O94907
Entrez Gene ID: (Human) 22943, (Rat) 293897
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
endoderm formation
mesoderm formation
hair follicle development
regulation of receptor internalization
heart induction
heart valve development
endocardial cushion development
endoderm development
learning or memory
positive regulation of gene expression
negative regulation of neuron projection development
Wnt signaling pathway
regulation of Wnt signaling pathway
negative regulation of Wnt signaling pathway
negative regulation of ossification
embryonic limb morphogenesis
negative regulation of BMP signaling pathway
forebrain development
response to retinoic acid
negative regulation of mesodermal cell fate specification
regulation of endodermal cell fate specification
negative regulation of apoptotic process
regulation of neuron apoptotic process
positive regulation of Wnt signaling pathway, calcium modulating pathway
positive regulation of JNK cascade
regulation of synapse organization
regulation of synaptic transmission, glutamatergic
canonical Wnt signaling pathway
limb development
head morphogenesis
face morphogenesis
negative regulation of SMAD protein import into nucleus
motor learning
negative regulation of canonical Wnt signaling pathway
Wnt signaling pathway involved in somitogenesis
synapse pruning
regulation of dopaminergic neuron differentiation
negative regulation of Wnt-Frizzled-LRP5/6 complex assembly
positive regulation of midbrain dopaminergic neuron differentiation
negative regulation of presynapse assembly
positive regulation of Wnt signaling pathway, planar cell polarity pathway
negative regulation of cardiac muscle cell differentiation
multicellular organism development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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