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          • Primary Antibodies ›
          • ARF6 Antibodies

          Invitrogen

          ARF6 Recombinant Rabbit Monoclonal Antibody (JF101-2)

          View all (12) ARF6 antibodies

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          Cite ARF6 Recombinant Rabbit Monoclonal Antibody (JF101-2)

          Additional Information:
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          • Antibody Testing Data (6)
          ARF6 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          ARF6 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          ARF6 Antibody (MA5-32558) in ICC/IF

          Immunocytochemical analysis of ARF6 in HepG2 cells using a ARF6 Monoclonal antibody (Product # MA5-32558) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          ARF6 Antibody in Immunocytochemistry (ICC/IF)
          ARF6 Antibody in Immunocytochemistry (ICC/IF)
          ARF6 Antibody in Immunocytochemistry (ICC/IF)
          ARF6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ARF6 Antibody in Western Blot (WB)
          ARF6 Antibody in Western Blot (WB)
          ARF6 Recombinant Rabbit Monoclonal Antibody (JF101-2)

          Product Details

          MA5-32558

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:500
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          JF101-2

          Immunogen

          Synthetic peptide within Human ARF6 aa 133-165.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2809835

          Product Specific Information

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          Target Information

          ARF6 (ADP-ribosylation factor 6) is a broadly expressed, differentially regulated GTPase that is present in cytoplasm and on both cell-surface and intracellular membranes and whose functions may include tissue-specific effects on vesicular trafficking during cellular differentiation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: ADP ribosylation factor 6; ADP-ribosylation factor 6; ARF6; DKFZp564M0264; small GTP binding protein; unnamed protein product

          View more View less

          Gene Aliases: AI788669; ARF6; AW496366

          View more View less

          UniProt ID: (Human) P62330, (Rat) P62332, (Mouse) P62331

          View more View less

          Entrez Gene ID: (Human) 382, (Rat) 79121, (Mouse) 11845

          View more View less

          Function(s)
          nucleotide binding GTPase activity obsolete small monomeric GTPase activity protein binding GTP binding hydrolase activity GDP binding thioesterase binding signaling adaptor activity protein N-terminus binding G-protein
          Process(es)
          mitotic cytokinesis liver development intracellular protein transport vesicle docking involved in exocytosis cell adhesion nervous system development regulation of neuron projection development positive regulation of neuron projection development protein transport vesicle-mediated transport cell differentiation positive regulation of actin filament polymerization cortical actin cytoskeleton organization endocytic recycling protein localization to cell surface regulation of Rac protein signal transduction protein localization to endosome negative regulation of receptor-mediated endocytosis synaptic vesicle endocytosis positive regulation of protein secretion cell division regulation of filopodium assembly positive regulation of keratinocyte migration regulation of dendritic spine development cellular response to diacyl bacterial lipopeptide protein localization to plasma membrane establishment of epithelial cell polarity ruffle assembly hepatocyte apoptotic process maintenance of postsynaptic density structure positive regulation of focal adhesion disassembly erythrocyte apoptotic process positive regulation of protein localization to plasma membrane positive regulation of mitotic cytokinetic process protein localization to cleavage furrow regulation of presynapse assembly cellular response to nerve growth factor stimulus negative regulation of protein localization to cell surface negative regulation of dendrite development small GTPase mediated signal transduction actin cytoskeleton organization ruffle organization myeloid cell apoptotic process positive regulation of establishment of protein localization to plasma membrane transport cell cycle regulation of toll-like receptor 4 signaling pathway
          It has to be done as per old AB suggested Products section.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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