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Invitrogen
PRKAR2B Recombinant Rabbit Monoclonal Antibody (JA11-69)
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FIGURE: 1 / 5
PRKAR2B Antibody (MA5-32662) in ICC/IF
Immunocytochemical analysis of PRKAR2B in SH-SY5Y cells using a PRKAR2B Monoclonal antibody (Product # MA5-32662) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-32662
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA11-69
Immunogen
Synthetic peptide within Human PKA 2 beta aa 369-418.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cAMP-dependent protein kinase type II-beta regulatory chain; cAMP-dependent protein kinase type II-beta regulatory subunit; H_RG363E19.2; protein kinase, cAMP-dependent, regulatory subunit type II beta; protein kinase, cAMP-dependent, regulatory, type II, beta; Type II beta regulatory subunit of cAMP-dependent protein kinase; unnamed protein product; WUGSC:H_RG363E19.2
Gene Aliases: AI451071; AW061005; Pkarb2; PKARIIbeta; PRKAR2; PRKAR2B; RATDNA; RII(beta); RII-BETA
UniProt ID: (Human) P31323, (Mouse) P31324, (Rat) P12369
Entrez Gene ID: (Human) 5577, (Mouse) 19088, (Rat) 24679
nucleotide binding
cAMP-dependent protein kinase inhibitor activity
protein binding
cAMP-dependent protein kinase regulator activity
protein kinase binding
protein domain specific binding
cAMP binding
ubiquitin protein ligase binding
protein kinase A catalytic subunit binding
protein-binding activity modulator
kinase modulator
fatty acid metabolic process
adenylate cyclase-activating G-protein coupled receptor signaling pathway
learning
intracellular signal transduction
modulation of synaptic transmission
response to antipsychotic drug
negative regulation of cAMP/PKA signal transduction
regulation of protein phosphorylation
regulation of protein kinase activity
negative regulation of cAMP-dependent protein kinase activity
response to clozapine
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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