Search
Invitrogen
Leptin Receptor Recombinant Rabbit Monoclonal Antibody (JA73-01)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 5
Leptin Receptor Antibody (MA5-32685) in ICC/IF
Immunocytochemistry analysis of Leptin Receptor in K-562 cells. Samples were incubated with Leptin Receptor recombinant monoclonal antibody (Product # MA5-32685) using a dilution of 1:100 in 1% BSA in PBST overnight at 4°C. Followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-... View More
Product Details
MA5-32685
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA73-01
Immunogen
Synthetic peptide within Human Leptin Receptor aa 1041-1090.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The leptin (OB-R) receptor is a member of the cytokine receptor superfamily which plays an important role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of the OB-R by the adipose tissue-secreted hormone, leptin, appears to activate downstream signaling events through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Leptin can homo-dimerize OB-R extracellular domains while downstream signaling events are initiated upon homo-dimerization of OB-R intracellular domains. Several isoforms of the OB-R which differ in length and signaling capabilities have been reported. RNA transcripts encoding the OB-R long form have been found in the choroid plexus and in the hypothalamus which has been proposed as a control center for satiety and energy expenditure. Mutations in either the mouse OB-R (db) or leptin (ob) genes have been shown to result in early-onset obesity. Metabolic abnormalities attributable to these genotypes include hypercorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered CNS activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B219; CD antigen CD295; CD295; CD295 antigen; cytokine receptor; HuB219; HuB219.2; LEP-R; Leptin receptor; Leptin receptor (fatty); leptin receptor gene-related protein; OB receptor; OB-R; splice variant form 12.1; Ob receptor; splice variant form 13.2; Ob receptor; splice variant form 6.4; Ob receptor; unnamed protein product
Gene Aliases: CD295; DB; diabetes; Fa; LEP-R; LEPR; Leprb; LEPRD; LEPROT; Modb1; OB-R; OB-RGRP; obese-like; obl; OBR
UniProt ID: (Human) P48357, (Rat) Q62959, (Mouse) P48356
Entrez Gene ID: (Human) 3953, (Rat) 24536, (Mouse) 16847
angiogenesis
glycogen metabolic process
gluconeogenesis
energy reserve metabolic process
phagocytosis
cell surface receptor signaling pathway
multicellular organism development
cholesterol metabolic process
positive regulation of cell proliferation
negative regulation of autophagy
glial cell proliferation
cytokine-mediated signaling pathway
sexual reproduction
T cell differentiation
leptin-mediated signaling pathway
glucose homeostasis
response to leptin
negative regulation of gluconeogenesis
regulation of bone remodeling
system development
regulation of transport
regulation of feeding behavior
energy homeostasis
receptor signaling pathway via STAT
bone growth
positive regulation of cold-induced thermogenesis
transport across blood-brain barrier
ovulation from ovarian follicle
response to hypoxia
generation of precursor metabolites and energy
G-protein coupled receptor signaling pathway
female pregnancy
response to nutrient
response to nicotine
eating behavior
positive regulation of MAPK cascade
response to estrogen
negative regulation of hydrolase activity
cellular response to organic substance
signal transduction
regulation of metabolic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support