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Invitrogen

PKC epsilon Recombinant Rabbit Monoclonal Antibody (JA38-21)

View all (28) PKC epsilon antibodies

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PKC epsilon Recombinant Rabbit Monoclonal Antibody (JA38-21)

Product Details

MA5-32715

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JA38-21

Immunogen

Synthetic peptide within Human PKC epsilon aa 561-610.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809992

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC125656; MGC125657; nPKC-epsilon; PKC e; Protein kinase C epsilon type; unnamed protein product

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Gene Aliases: 5830406C15Rik; nPKC-epsilon; PKC[e]; PKCE; Pkcea; PKCepsilon; PRKCE; R75156

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UniProt ID: (Human) Q02156, (Mouse) P16054, (Rat) P09216

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Entrez Gene ID: (Human) 5581, (Mouse) 18754, (Rat) 29340

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Function(s)
nucleotide binding actin monomer binding protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-independent protein kinase C activity protein binding ATP binding enzyme activator activity zinc ion binding kinase activity transferase activity enzyme binding receptor activator activity ethanol binding metal ion binding 14-3-3 protein binding protein serine kinase activity receptor binding transferase activity, transferring phosphorus-containing groups SH3 domain binding protein kinase binding non-receptor serine/threonine protein kinase protein modifying enzyme
Process(es)
MAPK cascade macrophage activation involved in immune response immune system process apoptotic process cell adhesion signal transduction toxin catabolic process positive regulation of epithelial cell migration positive regulation of fibroblast migration positive regulation of cell-substrate adhesion regulation of metal ion transport peptidyl-serine phosphorylation regulation of lipid metabolic process insulin secretion positive regulation of actin filament polymerization negative regulation of protein ubiquitination cell-substrate adhesion lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion positive regulation of synaptic transmission, GABAergic positive regulation of cytokinesis positive regulation of superoxide anion generation intracellular signal transduction locomotory exploration behavior TRAM-dependent toll-like receptor 4 signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis xenobiotic catabolic process positive regulation of I-kappaB kinase/NF-kappaB signaling response to morphine positive regulation of MAPK cascade positive regulation of lipid catabolic process regulation of release of sequestered calcium ion into cytosol cell division establishment of localization in cell synaptic transmission, GABAergic regulation of insulin secretion involved in cellular response to glucose stimulus mucus secretion positive regulation of mucus secretion cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of wound healing negative regulation of sodium ion transmembrane transport positive regulation of protein localization to plasma membrane regulation of cation transmembrane transport protein phosphorylation cell cycle chemosensory behavior negative regulation of mitochondrial membrane potential phosphorylation cellular response to platelet-derived growth factor stimulus negative regulation of apoptotic process positive regulation of catalytic activity regulation of peptidyl-tyrosine phosphorylation release of sequestered calcium ion into cytosol negative regulation of release of sequestered calcium ion into cytosol negative regulation of mitochondrial calcium ion concentration positive regulation of receptor activity positive regulation of cellular glucuronidation
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