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Invitrogen
CYP3A4 Recombinant Rabbit Monoclonal Antibody (JA11-33)
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FIGURE: 1 / 4
CYP3A4 Antibody (MA5-32718) in ICC/IF
Immunofluorescence analysis of CYP3A4 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CYP3A4 Recombinant Rabbit Monoclonal Antibody (JA11-33) (Product # MA5-32718) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plu... View More
Product Details
MA5-32718
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA11-33
Immunogen
Synthetic peptide within Human CYP3A4 aa 454-503.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase (sulfoxide-forming); Albendazole sulfoxidase; catalyzes oxidative metabolism of xenobiotics; Cholesterol 25-hydroxylase; CYP; CYPIIIA3; CYPIIIA4; cytochrome P-450 nifedipine oxidase; Cytochrome P450 3A3; Cytochrome P450 3A4; Cytochrome P450 HLp; Cytochrome P450 NF-25; cytochrome P450, family 3, subfamily A, polypeptide 4; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; Cytochrome P450-PCN1; glucocorticoid-inducible cytochrome P-450; glucocorticoid-inducible P450; MGC126680; Nifedipine oxidase; P450; P450-III, steroid inducible; P450-PCN1; Quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase; unnamed protein product
Gene Aliases: CP33; CP34; CYP3A; CYP3A3; CYP3A4; CYPIIIA3; CYPIIIA4; HLP; NF-25; P450C3; P450PCN1; VDDR3
UniProt ID: (Human) P08684
Entrez Gene ID: (Human) 1576
monooxygenase activity
steroid binding
iron ion binding
protein binding
steroid hydroxylase activity
retinoic acid 4-hydroxylase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
oxygen binding
enzyme binding
heme binding
vitamin D3 25-hydroxylase activity
caffeine oxidase activity
metal ion binding
quinine 3-monooxygenase activity
testosterone 6-beta-hydroxylase activity
1-alpha,25-dihydroxyvitamin D3 23-hydroxylase activity
anandamide 8,9 epoxidase activity
anandamide 11,12 epoxidase activity
anandamide 14,15 epoxidase activity
vitamin D 24-hydroxylase activity
estrogen 16-alpha-hydroxylase activity
estrogen 2-hydroxylase activity
1,8-cineole 2-exo-monooxygenase activity
lipid hydroxylation
lipid metabolic process
fatty acid metabolic process
steroid biosynthetic process
steroid catabolic process
xenobiotic metabolic process
steroid metabolic process
cholesterol metabolic process
androgen metabolic process
estrogen metabolic process
alkaloid catabolic process
monoterpenoid metabolic process
calcitriol biosynthetic process from calciol
xenobiotic catabolic process
vitamin D metabolic process
vitamin D catabolic process
retinol metabolic process
retinoic acid metabolic process
long-chain fatty acid biosynthetic process
aflatoxin metabolic process
oxidative demethylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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