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Invitrogen
FTO Recombinant Rabbit Monoclonal Antibody (JM98-30)
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FIGURE: 1 / 8
FTO Antibody (MA5-32785) in ICC/IF
Immunofluorescence analysis of Alpha-ketoglutarate-dependent dioxygenase FTO was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FTO Recombinant Rabbit Monoclonal Antibody (JM98-30) (Product # MA5-32785) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adso... View More
Product Details
MA5-32785
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JM98-30
Immunogen
Synthetic peptide within Human FTO aa 1-50.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
FTO is a novel protein and a member of the non-heme dioxygenase (Fe(II)-and 2-oxoglutarate-dependent dioxygenases) superfamily. Amino acid conservation patterns support this hypothesis and indicates that both 2-oxoglutarate and iron should be important for FTO function. Though not much is yet known about FTO, it is thought that the protein may somehow modify the activity of genes involved in metabolism and fat storage, which in turn may influence a person's risk of obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AlkB homolog 9; Alpha-ketoglutarate-dependent dioxygenase FTO; fat mass and obesity associated; Fat mass and obesity-associated protein; fatso; intragenic FTO exon 9 containing transcript; m6A(m)-demethylase FTO; MGC5149; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; tRNA N1-methyl adenine demethylase FTO; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO; unnamed protein product
Gene Aliases: ALKBH9; BMIQ14; FTO; GDFD; IFEX9; KIAA1752
UniProt ID: (Human) Q9C0B1
Entrez Gene ID: (Human) 79068
protein binding
ferrous iron binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
transferase activity
oxidative RNA demethylase activity
oxidative DNA demethylase activity
metal ion binding
dioxygenase activity
RNA N6-methyladenosine dioxygenase activity
tRNA demethylase activity
temperature homeostasis
DNA dealkylation involved in DNA repair
regulation of lipid storage
snRNA processing
regulation of multicellular organism growth
RNA repair
regulation of respiratory system process
adipose tissue development
mRNA destabilization
regulation of white fat cell proliferation
regulation of brown fat cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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