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          • ARSA Antibodies

          Invitrogen

          Arylsulfatase A Monoclonal Antibody (4C10)

          View all (20) ARSA antibodies

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          Cite Arylsulfatase A Monoclonal Antibody (4C10)

          Additional Information:
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          • Antibody Testing Data (6)
          Arylsulfatase A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          Arylsulfatase A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          Arylsulfatase A Antibody (MA5-33005) in IHC (P)

          Immunohistochemical analysis of ARSA in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-ARSA antibody (Product # MA5-33005) overnight at 4°C. Biotinylated goat anti-rabbit IgG wa... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Arylsulfatase A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Arylsulfatase A Antibody in Western Blot (WB)
          Arylsulfatase A Antibody in Western Blot (WB)
          Arylsulfatase A Antibody in Flow Cytometry (Flow)
          Arylsulfatase A Antibody in Flow Cytometry (Flow)
          Arylsulfatase A Antibody in Flow Cytometry (Flow)
          Arylsulfatase A Monoclonal Antibody (4C10)

          Product Details

          MA5-33005

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          0.5-1 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          4C10

          Immunogen

          A synthetic peptide corresponding to a sequence at the C-terminus of human ARSA (454-482aa QALKQLQLLKAQLDAAVTFGPSQVARGED).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          0.05mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2802639

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: rat testis tissue, rat liver tissue, rat brain tissue, rat lung tissue, mouse testis tissue, mouse liver tissue, mouse brain tissue, mouse lung tissue, mouse HEPA1-6 whole cell, human A375 whole cell, human A549 whole cell, human SMMC-7721 whole cell. IHC: human lung cancer tissue. Flow: ANA-1 cell, Raji cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy, a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: ARSA; ARSA-I; ARSA1; Arylsulfatase A; ASA; ASNA-I; ATPase ASNA1; Cerebroside-sulfatase; EC 3.1.6.8; epididymis secretory sperm binding protein; GET3; hASNA-I; TRC40; unnamed protein product

          View more View less

          Gene Aliases: ARSA; As-2; AS-A; As2; ASA; AW212749; MLD; TISP73

          View more View less

          UniProt ID: (Human) P15289, (Mouse) P50428

          View more View less

          Entrez Gene ID: (Human) 410, (Rat) 315222, (Mouse) 11883

          View more View less

          Function(s)
          arylsulfatase activity cerebroside-sulfatase activity calcium ion binding protein binding sulfuric ester hydrolase activity hydrolase activity metal ion binding catalytic activity hydrolase
          Process(es)
          lipid metabolic process autophagy binding of sperm to zona pellucida central nervous system development response to nutrient response to pH response to estrogen response to ethanol response to methylmercury metabolic process
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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