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Invitrogen

Phospho-STAT3 (Ser727) Recombinant Rabbit Monoclonal Antibody (2A9)

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Phospho-STAT3 (Ser727) Recombinant Rabbit Monoclonal Antibody (2A9)

Product Details

MA5-33199

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100
-

ELISA (ELISA)

Assay-Dependent
-

Immunoprecipitation (IP)

1:200-1:1,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2A9

Immunogen

A synthesized peptide derived from human Phospho-STAT3 (S727)
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Amount

150 µg

Purification

Protein G, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2812015

Target Information

STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acute-phase response factor; DNA-binding protein APRF; FLJ20882; MGC16063; STAT; Stat3; unnamed protein product

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Gene Aliases: ADMIO; ADMIO1; APRF; HIES

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UniProt ID: (Human) P40763

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Entrez Gene ID: (Human) 6774

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding RNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding receptor binding protein binding protein kinase binding protein phosphatase binding chromatin DNA binding CCR5 chemokine receptor binding glucocorticoid receptor binding signaling adaptor activity identical protein binding protein homodimerization activity sequence-specific DNA binding protein dimerization activity RNA polymerase II sequence-specific DNA binding transcription factor binding primary miRNA binding lncRNA binding DNA-binding transcription factor binding protein sequestering activity RNA sequestering activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter temperature homeostasis response to hypoxia eye photoreceptor cell differentiation regulation of cytokine production response to ischemia regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein import into nucleus defense response acute-phase response inflammatory response immune response signal transduction transforming growth factor beta receptor signaling pathway JAK-STAT cascade nervous system development positive regulation of cell proliferation negative regulation of autophagy positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of hydrogen peroxide biosynthetic process cytokine-mediated signaling pathway cell differentiation positive regulation of cell migration intracellular receptor signaling pathway response to estradiol positive regulation of interleukin-1 beta production positive regulation of interleukin-10 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production cellular response to hormone stimulus leptin-mediated signaling pathway response to cytokine interleukin-15-mediated signaling pathway interleukin-2-mediated signaling pathway interleukin-9-mediated signaling pathway interleukin-11-mediated signaling pathway interleukin-23-mediated signaling pathway oncostatin-M-mediated signaling pathway regulation of cell proliferation glucose homeostasis eating behavior positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling response to peptide hormone protein kinase B signaling negative regulation of neuron apoptotic process cellular response to leptin stimulus response to leptin response to ethanol positive regulation of erythrocyte differentiation positive regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of mitochondrial membrane permeability astrocyte differentiation leukemia inhibitory factor signaling pathway negative regulation of inflammatory response positive regulation of inflammatory response positive regulation of phagocytosis modulation of synaptic transmission positive regulation of multicellular organismal process regulation of cell cycle radial glial cell differentiation regulation of feeding behavior growth hormone receptor signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway interleukin-6-mediated signaling pathway ciliary neurotrophic factor-mediated signaling pathway T-helper 17 type immune response T-helper 17 cell lineage commitment positive regulation of extracellular matrix disassembly energy homeostasis receptor signaling pathway via STAT postsynapse to nucleus signaling pathway negative regulation of inflammatory response to wounding interleukin-10-mediated signaling pathway negative regulation of cytokine production involved in inflammatory response positive regulation of cytokine production involved in inflammatory response regulation of cellular response to hypoxia positive regulation of growth factor dependent skeletal muscle satellite cell proliferation positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of primary miRNA processing positive regulation of ATP biosynthetic process
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