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FIGURE: 1 / 10
RXRA Antibody (MA5-34806) in ICC/IF
Immunofluorescence of RXR-alpha was performed using HCT116 wild-type and RXR-alpha KO cells that were transfected with a green or a far-red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) or methanol for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the RXR-alpha rec... View More
Product Details
MA5-34806
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JG99-38
Immunogen
Recombinant protein corresponding to Human Retinoid X Receptor alpha aa 1-50.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: MCF-7, rat liver tissue, human esophagus tissue.
Target Information
Retinoic Acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells. The Retinoid X Receptors (RXRs) family (RXR alpha, beta, and gamma), preferentially bind 9-cis -RA and regulate gene transcription by forming heterodimers with a second family of RA receptors. RAs have been suggested to potentially play a therapeutic role in cervical cancer. RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Nuclear receptor subfamily 2 group B member 1; ORF-3; Retinoic acid receptor RXR-alpha; retinoid X nuclear receptor alpha; retinoid X receptor; Retinoid X receptor alpha; rxr alpha; unnamed protein product
Gene Aliases: NR2B1; RXR-alpha; RXRA; RXRalpha
UniProt ID: (Human) P19793
Entrez Gene ID: (Human) 6256
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription cofactor binding
retinoic acid binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
enzyme binding
peptide binding
identical protein binding
vitamin D receptor binding
sequence-specific DNA binding
retinoic acid-responsive element binding
metal ion binding
DBD domain binding
LBD domain binding
vitamin D response element binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
positive regulation of thyroid hormone receptor signaling pathway
regulation of transcription, DNA-templated
nervous system development
hormone-mediated signaling pathway
positive regulation of cholesterol efflux
cell differentiation
monocyte differentiation
positive regulation of bone mineralization
intracellular steroid hormone receptor signaling pathway
response to retinoic acid
peroxisome proliferator activated receptor signaling pathway
mRNA transcription from RNA polymerase II promoter
positive regulation of lipid metabolic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
retinoic acid receptor signaling pathway
cell maturation
positive regulation of vitamin D receptor signaling pathway
cellular response to low-density lipoprotein particle stimulus
nucleus-associated proteasomal ubiquitin-dependent protein catabolic process
positive regulation of lipoprotein transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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