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Invitrogen
E2F1 Recombinant Rabbit Monoclonal Antibody (5Z5U3)
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FIGURE: 1 / 3
E2F1 Antibody (MA5-35306) in WB
Western blot analysis of E2F1 in extracts of various cell lines. Samples were incubated with E2F1 Monoclonal antibody (Product # MA5-35306) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
MA5-35306
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5Z5U3
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 338-437 of human E2F1 (Q01094)
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Amount
40 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PPSSLTTDPS QSLLSLEQEP LLSRMGSLRA PVDEDRLSPL VAADSLLEHV REDFSGLLPE EFISLSPPHE ALDYHFGLEE GEGIRDLFDC DFGDLTPLDF
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; retinoblastoma-binding protein 3 (E2F-like); transcription factor E2F like protein; Transcription factor E2F1
Gene Aliases: E2F-1; E2F1; mKIAA4009; RBAP1; RBBP3; RBP3
UniProt ID: (Human) Q01094, (Mouse) Q61501, (Rat) O09139
Entrez Gene ID: (Human) 1869, (Mouse) 13555, (Rat) 399489
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein kinase binding
sequence-specific DNA binding
protein dimerization activity
binding, bridging
DNA-binding transcription factor binding
sequence-specific double-stranded DNA binding
core promoter binding
transcription factor binding
transcription regulatory region DNA binding
RNA metabolism protein
nucleic acid metabolism protein
general transcription factor
DNA damage checkpoint
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
apoptotic process
spermatogenesis
intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of gene expression
forebrain development
response to lipopolysaccharide
positive regulation of apoptotic process
anoikis
negative regulation of DNA binding
negative regulation of fat cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
mRNA stabilization
regulation of cell cycle
positive regulation of glial cell proliferation
negative regulation of fat cell proliferation
cellular response to fatty acid
cellular response to hypoxia
cellular response to xenobiotic stimulus
intrinsic apoptotic signaling pathway by p53 class mediator
lens fiber cell apoptotic process
cellular response to nerve growth factor stimulus
regulation of G1/S transition of mitotic cell cycle
cell cycle
negative regulation of transcription involved in G1/S transition of mitotic cell cycle
G1/S transition of mitotic cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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