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Invitrogen

SP1 Recombinant Rabbit Monoclonal Antibody (5I3A8)

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Product Details

MA5-35331

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000
-

Immunocytochemistry (ICC/IF)

1:200-1:1,000
-

ELISA (ELISA)

1 µg/mL
-

ChIP assay (ChIP)

5 µg antibody for 10 µg-15 µg of Chromatin
View 1 publication 1 publication

ChIP-sequencing (ChIP-Seq)

1:50-1:100
-

CUT&RUN (CUT&RUN)

10^5 cells /1 µg
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5I3A8

Immunogen

Synthetic peptide.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Amount

150 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849233

Target Information

SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HGNC:11205; Specificity protein 1; trans-acting transcription factor 1; transcription factor; transcription factor Sp-1; Transcription factor Sp1; transcriptional activator protein; unnamed protein product

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Gene Aliases: 1110003E12Rik; AA450830; AI845540; SP1; Sp1-1; TSFP1

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UniProt ID: (Human) P08047, (Mouse) O89090, (Rat) Q01714

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Entrez Gene ID: (Human) 6667, (Mouse) 20683, (Rat) 24790

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding histone acetyltransferase binding identical protein binding protein homodimerization activity histone deacetylase binding bHLH transcription factor binding sequence-specific DNA binding metal ion binding binding, bridging RNA polymerase II sequence-specific DNA binding transcription factor binding sequence-specific double-stranded DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding nucleic acid binding protein C-terminus binding transcription factor binding enhancer binding transcription regulatory region DNA binding HMG box domain binding C2H2 zinc finger transcription factor
Process(es)
regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter positive regulation of gene expression cellular response to insulin stimulus response to hydroperoxide cellular response to zinc ion starvation positive regulation of blood vessel endothelial cell migration positive regulation by host of viral transcription positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process cellular response to estrogen stimulus positive regulation of beta-amyloid formation cellular response to wortmannin positive regulation of hydrogen sulfide biosynthetic process positive regulation of vascular endothelial cell proliferation positive regulation of apoptotic signaling pathway ossification in utero embryonic development trophectodermal cell differentiation liver development embryonic placenta development transcription, DNA-templated transcription from RNA polymerase II promoter megakaryocyte differentiation lung development enucleate erythrocyte differentiation embryonic camera-type eye morphogenesis embryonic skeletal system development embryonic process involved in female pregnancy definitive hemopoiesis
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