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Invitrogen
MYLK Recombinant Rabbit Monoclonal Antibody (1J2U2)
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FIGURE: 1 / 1
MYLK Antibody (MA5-35409) in WB
Western blot analysis of MYLK in extracts of various cell lines. Samples were incubated with MYLK Monoclonal antibody (Product # MA5-35409) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
MA5-35409
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1J2U2
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1800-1900 of human MYLK (Q15746)
Conjugate
Form
Liquid
Concentration
0.65 mg/mL
Amount
65 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: VAEEKPHVKP YFSKTIRDLE VVEGSAARFD CKIEGYPDPE VVWFKDDQSI RESRHFQIDY DEDGNCSLII SDVCGDDDAK YTCKAVNSLG EATCTAELIV E
Target Information
MYLK (MLCK) is a serine/threonine kinase that phosphorylates myosin regulatory light chains to assist in the production of contractile activity (myosin-actin interaction). MLCK is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Kinase-related protein; KRP; MLCK; Myosin light chain kinase, smooth muscle; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase; Telokin; unnamed protein product
Gene Aliases: AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210; MMIHS; MMIHS1; MSTP083; MYLK; MYLK-L; MYLK1; smMLCK
UniProt ID: (Human) Q15746
Entrez Gene ID: (Human) 4638, (Rat) 288057
nucleotide binding
actin binding
protein kinase activity
protein serine/threonine kinase activity
myosin light chain kinase activity
protein binding
calmodulin binding
ATP binding
kinase activity
transferase activity
metal ion binding
scaffold protein binding
calmodulin-dependent protein kinase activity
enzyme binding
protein phosphorylation
smooth muscle contraction
actin filament organization
tonic smooth muscle contraction
positive regulation of cell migration
bleb assembly
positive regulation of calcium ion transport
aorta smooth muscle tissue morphogenesis
cellular hypotonic response
positive regulation of wound healing
muscle organ development
regulation of synaptic vesicle endocytosis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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