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Invitrogen
S1P1 Recombinant Rabbit Monoclonal Antibody (9R8N3)
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FIGURE: 1 / 1
S1P1 Antibody (MA5-35431) in WB
Western blot analysis of S1P1 in extracts of various cell lines. Samples were incubated with S1P1 Monoclonal antibody (Product # MA5-35431) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
MA5-35431
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9R8N3
Immunogen
Synthetic peptide
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Amount
33 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
S1P1 is a G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that is coupled to the G(i) subclass of heteromeric G proteins. S1P1 signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. It plays an important role in cell migration via its role in the reorganization of the actin cytoskeleton and formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. S1P1 is required for normal chemotaxis toward sphingosine 1-phosphate, normal embryonic heart development, and normal cardiac morphogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD antigen CD363; CD363; CD363 antigen; EDG1; EDG1 (Edg1); Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation protein (edg-1); endothelial differentiation sphingolipid G-protein-coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; Lysophospholipid receptor B1; S1P receptor 1; S1P receptor Edg-1; S1P1; similar to spleen endothelial differentiation gene EDG-1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; sphingosine 1-phosphate receptor EDG1; unnamed protein product
Gene Aliases: AI849002; CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; Lpb1; S1p; S1P1; S1PR1
UniProt ID: (Human) P21453, (Rat) P48303, (Mouse) O08530
Entrez Gene ID: (Human) 1901, (Rat) 29733, (Mouse) 13609
angiogenesis
blood vessel maturation
cardiac muscle tissue growth involved in heart morphogenesis
sphingosine-1-phosphate signaling pathway
chemotaxis
cell adhesion
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
brain development
cell proliferation
positive regulation of cell proliferation
cell migration
transmission of nerve impulse
lamellipodium assembly
actin cytoskeleton organization
regulation of cell adhesion
neuron differentiation
positive regulation of cell migration
regulation of bone mineralization
leukocyte chemotaxis
regulation of bone resorption
endothelial cell differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
positive regulation of positive chemotaxis
negative regulation of stress fiber assembly
heart trabecula morphogenesis
T cell migration
actin cytoskeleton reorganization
positive regulation of GTPase activity
positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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