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Invitrogen
SKP2 Recombinant Rabbit Monoclonal Antibody (2L0K1)
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FIGURE: 1 / 8
SKP2 Antibody (MA5-35433) in ICC/IF
Immunofluorescence analysis of SKP2 was performed using 70% confluent log phase BeWo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SKP2 Recombinant Rabbit Monoclonal Antibody (ARC0886) (Product # MA5-35433) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor P... View More
Product Details
MA5-35433
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2L0K1
Immunogen
Synthetic peptide
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Amount
80 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK2/cyclin A-associated protein p45; cyclin A/CDK2-associated protein p45; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; F-box/WD-40 protein 1; FWD1; MGC1366; OTTHUMP00000221389; p45skp2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2 (p45); S-phase kinase-associated protein 2, E3 ubiquitin protein ligase; unnamed protein product
Gene Aliases: 4930500A04Rik; FBL1; FBXL1; FLB1; FWD1; p45; RGD1562456; SKP2
UniProt ID: (Human) Q13309, (Mouse) Q9Z0Z3
Entrez Gene ID: (Human) 6502, (Rat) 294790, (Mouse) 27401
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
protein polyubiquitination
ubiquitin-dependent protein catabolic process
phosphorylation
positive regulation of intracellular estrogen receptor signaling pathway
regulation of apoptotic process
positive regulation of smooth muscle cell proliferation
regulation of cell cycle
cellular response to cell-matrix adhesion
positive regulation of protein polyubiquitination
DNA double-strand break processing
immune system process
protein ubiquitination
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
innate immune response
defense response to virus
protein K63-linked ubiquitination
protein K48-linked ubiquitination
positive regulation of double-strand break repair via homologous recombination
protein localization to site of double-strand break
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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