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Invitrogen
SMC1 Recombinant Rabbit Monoclonal Antibody (3M5E3)
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FIGURE: 1 / 7
SMC1 Antibody (MA5-35558) in ICC/IF
Immunofluorescence analysis of SMC1A/SMC1B was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SMC1 Recombinant Rabbit Monoclonal Antibody (ARC1095) (Product # MA5-35558) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor... View More
Product Details
MA5-35558
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3M5E3
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1134-1233 of human SMC1 (Q14683)
Conjugate
Form
Liquid
Concentration
0.21 mg/mL
Amount
21 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: TVAALALLFA IHSYKPAPFF VLDEIDAALD NTNIGKVANY IKEQSTCNFQ AIVISLKEEF YTKAESLIGV YPEQGDCVIS KVLTFDLTKY PDANPNPNEQ
Target Information
Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromo proteins are conserved in eukaryotes and lead to mitotic chromosome segregatior defects. This suggests a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. Mutations in the gene can lead to Cornelia de Lange syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chromosome segregation protein SmcB; DKFZp686L19178; epididymis secretory sperm binding protein; KIAA0178; MGC138332; mitosis-specific chromosome segregation protein like protein beta; mitosis-specific chromosome segregation protein SMC1 homolog; RP6-29D12.1; Sb1.8; segregation of mitotic chromosomes 1; segregation of mitotic chromosomes b; SM1A; SMC (segregation of mitotic chromosomes 1)-like 1; SMC protein 1A; SMC protein 1B; SMC-1-alpha; SMC-1-beta; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 2; SMC1alpha protein; structural maintenace of chromosomes 1B; Structural maintenance of chromosomes protein 1A; Structural maintenance of chromosomes protein 1B; unnamed protein product
Gene Aliases: 5830426I24Rik; CDLS2; DEE85; DXS423E; EIEE85; KIAA0178; mKIAA0178; SB1.8; SMC-1A; SMC-1B; SMC1; SMC1A; SMC1alpha; SMC1B; SMC1BETA; SMC1L1; SMC1L2; SMCB
UniProt ID: (Human) Q14683, (Human) Q8NDV3, (Mouse) Q9CU62, (Mouse) Q920F6
Entrez Gene ID: (Human) 8243, (Human) 27127, (Mouse) 24061, (Mouse) 140557
sister chromatid cohesion
chromosome organization
meiotic cell cycle
DNA repair
cellular response to DNA damage stimulus
cell cycle
mitotic sister chromatid cohesion
mitotic nuclear division
mitotic cell cycle checkpoint
meiotic nuclear division
stem cell population maintenance
negative regulation of DNA endoreduplication
signal transduction in response to DNA damage
cell division
mitotic sister chromatid segregation
response to radiation
establishment of mitotic sister chromatid cohesion
establishment of meiotic sister chromatid cohesion
somatic stem cell population maintenance
response to DNA damage checkpoint signaling
mitotic spindle assembly
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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