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          • Primary Antibodies ›
          • GATA2/GATA3 Antibodies

          Invitrogen

          GATA2/GATA3 Recombinant Rabbit Monoclonal Antibody (1K8H6)

          View all (5) GATA2/GATA3 antibodies

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          Cite GATA2/GATA3 Recombinant Rabbit Monoclonal Antibody (1K8H6)

          Additional Information:
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          • Antibody Testing Data (2)
          GATA2/GATA3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          GATA2/GATA3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          GATA2/GATA3 Antibody (MA5-35637) in WB

          Western blot analysis of GATA2/GATA3 in extracts of various cell lines. Samples were incubated with GATA2/GATA3 Monoclonal antibody (Product # MA5-35637) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          GATA2/GATA3 Antibody in Western Blot (WB)
          GATA2/GATA3 Antibody in Western Blot (WB)
          GATA2/GATA3 Recombinant Rabbit Monoclonal Antibody (1K8H6)

          Product Details

          MA5-35637

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          1K8H6

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human GATA2/3 (NP_1160272)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.5 mg/mL

          Amount

          150 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.05% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2849537

          Product Specific Information

          Immunogen sequence: MEVAPEQPRW MAHPAVLNAQ HPDSHHPGLA HNYMEPAQLL PPDEVDVFFN HLDSQGNPYY ANPAHARARV SYSPAHARLT GGQMCRPHLL HSPGLPWLDG GKAALSAAAA HHHNPWTVSP FSKTPLHPSA AGGPGGPLSV YPGAGGGSGG GSGSSVASLT PTAAHSGSHL FGFPPTPPKE VSPDPSTTGA ASPASSSAGG SAARGEDKDG

          Target Information

          The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Bioinformatics

          Protein Aliases: Endothelial transcription factor GATA-2; GATA-3; GATA-binding factor 3; GATA-binding protein 2; GATA-binding protein 3; GATA-bindning protein 2; gata2 {ECO:0000312|EMBL:AAK51128.1}; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; unnamed protein product

          View more View less

          Gene Aliases: DCML; Gata-2; Gata-3; GATA2; GATA3; HDR; HDRS; IMD21; jal; MONOMAC; NFE1B

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          UniProt ID: (Human) P23769, (Human) P23771, (Mouse) O09100, (Rat) Q924Y4, (Mouse) P23772

          View more View less

          Entrez Gene ID: (Human) 2624, (Human) 2625, (Mouse) 14461, (Rat) 25159, (Mouse) 14462, (Rat) 85471

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding sequence-specific DNA binding metal ion binding RNA polymerase II sequence-specific DNA binding transcription factor binding C2H2 zinc finger domain binding sequence-specific double-stranded DNA binding transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding interleukin-2 receptor binding identical protein binding E-box binding HMG box domain binding histone methyltransferase binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding enhancer sequence-specific DNA binding transcription factor binding RNA polymerase II core promoter sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator activity transcription regulatory region DNA binding protein dimerization activity DNA-binding transcription factor
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter angiogenesis urogenital system development cell fate determination neuron migration embryonic placenta development glandular epithelial cell differentiation glandular epithelial cell maturation hematopoietic progenitor cell differentiation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter phagocytosis positive regulation of cytosolic calcium ion concentration neuroblast proliferation negative regulation of neuroblast proliferation anatomical structure morphogenesis positive regulation of gene expression negative regulation of gene expression regulation of primitive erythrocyte differentiation ventral spinal cord interneuron differentiation cell differentiation in hindbrain commitment of neuronal cell to specific neuron type in forebrain central nervous system neuron differentiation central nervous system neuron development pituitary gland development myeloid cell differentiation cell differentiation neuron differentiation response to lipid somatic stem cell population maintenance eosinophil fate commitment inner ear morphogenesis neuron maturation positive regulation of mast cell degranulation positive regulation of blood vessel endothelial cell migration cell fate commitment fat cell differentiation negative regulation of myeloid cell differentiation positive regulation of erythrocyte differentiation negative regulation of macrophage differentiation positive regulation of megakaryocyte differentiation positive regulation of neuron differentiation negative regulation of Notch signaling pathway positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter neuron fate commitment system development homeostasis of number of cells within a tissue positive regulation of phagocytosis regulation of developmental process brown fat cell differentiation positive regulation of phagocytosis, engulfment thyroid-stimulating hormone-secreting cell differentiation definitive hemopoiesis semicircular canal development vascular wound healing neuroendocrine cell differentiation neural precursor cell proliferation hematopoietic stem cell homeostasis positive regulation of cell migration involved in sprouting angiogenesis cochlea development GABAergic neuron differentiation negative regulation of hematopoietic progenitor cell differentiation positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of brown fat cell differentiation positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis negative regulation of neural precursor cell proliferation negative regulation of endothelial cell apoptotic process regulation of forebrain neuron differentiation cell morphogenesis in utero embryonic development cell activation type IV hypersensitivity regulation of cytokine production positive regulation of cytokine production kidney development mesonephros development lens development in camera-type eye immune system process immune system development pro-T cell differentiation aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development chromatin remodeling defense response inflammatory response humoral immune response signal transduction nervous system development axon guidance cell proliferation negative regulation of cell proliferation male gonad development response to xenobiotic stimulus response to virus post-embryonic development positive regulation of signal transduction response to gamma radiation gene expression positive regulation of endothelial cell migration negative regulation of epithelial to mesenchymal transition regulation of neuron projection development T cell differentiation erythrocyte differentiation macrophage differentiation regulation of epithelial cell differentiation TOR signaling negative regulation of interferon-gamma production negative regulation of interleukin-2 production positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production T cell differentiation in thymus negative regulation of mammary gland epithelial cell proliferation embryonic hemopoiesis cellular response to interferon-alpha ureter maturation parathyroid hormone secretion norepinephrine biosynthetic process protein kinase B signaling regulation of neuron apoptotic process ear development response to estrogen thymic T cell selection T-helper 2 cell differentiation innate immune response response to ethanol positive regulation of T cell differentiation positive regulation of cell differentiation regulation of T-helper cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated cell maturation sympathetic nervous system development thymus development embryonic organ development developmental growth anatomical structure formation involved in morphogenesis homeostasis of number of cells negative regulation of inflammatory response T cell receptor signaling pathway cartilage development positive regulation of protein kinase B signaling parathyroid gland development pharyngeal system development uterus development canonical Wnt signaling pathway mesenchymal to epithelial transition mast cell differentiation ureteric bud formation cellular response to cytokine stimulus cellular response to interleukin-4 cellular response to tumor necrosis factor otic vesicle development cellular response to BMP stimulus positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation ureter morphogenesis lymphocyte migration regulation of establishment of cell polarity negative regulation of cell motility positive regulation of T-helper 2 cell cytokine production negative regulation of cell proliferation involved in mesonephros development positive regulation of thyroid hormone generation positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation transcription, DNA-templated hemopoiesis regulation of histone acetylation mast cell degranulation negative regulation of fat cell differentiation negative regulation of fat cell proliferation phosphatidylinositol 3-kinase signaling regulation of cytokine biosynthetic process regulation of CD4-positive, alpha-beta T cell differentiation regulation of histone H3-K4 methylation regulation of histone H3-K27 methylation canonical Wnt signaling pathway involved in metanephric kidney development renal system development interleukin-4 secretion interferon-gamma secretion positive regulation of interleukin-5 secretion positive regulation of interleukin-13 secretion response to drug
          It has to be done as per old AB suggested Products section.

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