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Invitrogen
PHOX2B Recombinant Rabbit Monoclonal Antibody (4W5J8)
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FIGURE: 1 / 1
PHOX2B Antibody (MA5-35695) in WB
Western blot analysis of PHOX2B in extracts of various cell lines. Samples were incubated with PHOX2B Monoclonal antibody (Product # MA5-35695) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
MA5-35695
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4W5J8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 215-314 of human PHOX2B (NP_0039152)
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Amount
150 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: GGGPSPAGAP GAAGPGGPGG EPGKGGAAAA AAAAAAAAAA AAAAAAGGLA AAGGPGQGWA PGPGPITSIP DSLGGPFASV LSSLQRPNGA KAALVKSSMF
Target Information
The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dilated pupils 1; GENA 269; NBPhox; neuroblastoma paired-type homeobox protein; Neuroblastoma Phox; paired like homeobox2B; paired mesoderm homeobox 2b; Paired mesoderm homeobox protein 2B; Paired-like homeobox 2B; PHOX2B homeodomain protein
Gene Aliases: CCHS; Dilp1; NBLST2; NBPhox; PHOX2B; PMX2B
UniProt ID: (Human) Q99453, (Mouse) O35690
Entrez Gene ID: (Human) 8929, (Mouse) 18935
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
sequence-specific DNA binding
homeodomain transcription factor
neuron migration
regulation of respiratory gaseous exchange by neurological system process
noradrenergic neuron differentiation
noradrenergic neuron development
brainstem development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cell proliferation
glial cell differentiation
regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
response to activity
cell differentiation in hindbrain
medullary reticular formation development
hindbrain tangential cell migration
neuron differentiation
skeletal muscle cell differentiation
type B pancreatic cell proliferation
negative regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
cell development
autonomic nervous system development
enteric nervous system development
sympathetic nervous system development
parasympathetic nervous system development
neuron development
inner ear development
efferent axon development in a lateral line nerve
membrane depolarization
respiratory system development
retrotrapezoid nucleus neuron differentiation
sympathetic ganglion development
cellular response to carbon dioxide
dopaminergic neuron differentiation
cellular response to BMP stimulus
motor neuron migration
positive regulation of cold-induced thermogenesis
neural crest cell migration involved in autonomic nervous system development
negative regulation of type B pancreatic cell proliferation
transcription from RNA polymerase II promoter
multicellular organism development
negative regulation of cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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