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          • Primary Antibodies ›
          • mTOR Antibodies

          Invitrogen

          Phospho-mTOR (Ser2448) Recombinant Rabbit Monoclonal Antibody (4O6A9)

          1 Reference
          View all (85) mTOR antibodies

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          Cite Phospho-mTOR (Ser2448) Recombinant Rabbit Monoclonal Antibody (4O6A9)

          Additional Information:
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          • Antibody Testing Data (2)
          Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Phospho-mTOR (Ser2448) Antibody (MA5-35832) in WB

          Western blot analysis of Phospho-mTOR (Ser2448) in extracts of 293T cells. Samples were incubated with Phospho-mTOR (Ser2448) Monoclonal antibody (Product # MA5-35832) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. 293T cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight or treated by IGF-1 (50 ng/mL) at... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
          Phospho-mTOR (Ser2448) Antibody in Western Blot (WB)
          Phospho-mTOR (Ser2448) Recombinant Rabbit Monoclonal Antibody (4O6A9)

          Product Details

          MA5-35832

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 1 publication 1 publication

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          4O6A9

          Immunogen

          A synthetic phosphorylated peptide around S2448 of human mTOR (P42345)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.22 mg/mL

          Amount

          22 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.05% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2849732

          Product Specific Information

          Immunogen sequence: TDSYS

          Target Information

          FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; Mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; mTORC1; Rapamycin and FKBP12 target 1; rapamycin and FKBP12 target-1 protein; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1; Serine/threonine-protein kinase mTOR; Tyrosine-protein kinase mTOR; unnamed protein product

          View more View less

          Gene Aliases: 2610315D21Rik; AI327068; flat; FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; SKS

          View more View less

          UniProt ID: (Human) P42345, (Mouse) Q9JLN9, (Rat) P42346

          View more View less

          Entrez Gene ID: (Human) 2475, (Mouse) 56717, (Rat) 56718

          View more View less

          Function(s)
          nucleotide binding inositol hexakisphosphate binding RNA polymerase III type 1 promoter sequence-specific DNA binding RNA polymerase III type 2 promoter sequence-specific DNA binding RNA polymerase III type 3 promoter sequence-specific DNA binding TFIIIC-class transcription factor binding protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity protein binding ATP binding kinase activity transferase activity identical protein binding ribosome binding ion channel binding macromolecular complex binding phosphoprotein binding protein serine kinase activity RNA polymerase III type 1 promoter DNA binding RNA polymerase III type 2 promoter DNA binding RNA polymerase III type 3 promoter DNA binding drug binding transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor protein domain specific binding protein dimerization activity non-receptor serine/threonine protein kinase protein modifying enzyme
          Process(es)
          autophagosome assembly regulation of cell growth cytoplasmic translation T-helper 1 cell lineage commitment heart morphogenesis heart valve morphogenesis energy reserve metabolic process 'de novo' pyrimidine nucleobase biosynthetic process regulation of translational initiation ubiquitin-dependent protein catabolic process inflammatory response cellular response to DNA damage stimulus cytoskeleton organization lysosome organization germ cell development positive regulation of cell proliferation insulin receptor signaling pathway regulation of cell size cellular response to starvation response to heat response to virus post-embryonic development positive regulation of biosynthetic process regulation of autophagy negative regulation of autophagy positive regulation of autophagy positive regulation of lamellipodium assembly positive regulation of epithelial to mesenchymal transition positive regulation of myotube differentiation macroautophagy regulation of macroautophagy negative regulation of macroautophagy regulation of lipid metabolic process neuronal action potential cell projection organization positive regulation of cell growth positive regulation of actin filament polymerization SCF-dependent proteasomal ubiquitin-dependent protein catabolic process T cell costimulation ruffle organization regulation of myelination protein destabilization response to nutrient levels cellular response to nutrient levels cellular response to nutrient TOR signaling response to insulin cellular response to insulin stimulus regulation of actin cytoskeleton organization calcineurin-NFAT signaling cascade cellular response to amino acid starvation multicellular organism growth TORC1 signaling TORC2 signaling regulation of circadian rhythm negative regulation of apoptotic process response to amino acid anoikis protein kinase B signaling positive regulation of cell differentiation regulation of osteoclast differentiation positive regulation of translation negative regulation of cell size positive regulation of glycolytic process negative regulation of innate immune response positive regulation of transcription from RNA polymerase III promoter negative regulation of translational initiation positive regulation of translational initiation negative regulation of insulin receptor signaling pathway positive regulation of lipid biosynthetic process behavioral response to pain oligodendrocyte differentiation positive regulation of oligodendrocyte differentiation cardiac muscle tissue development protein stabilization voluntary musculoskeletal movement positive regulation of multicellular organismal process positive regulation of stress fiber assembly positive regulation of keratinocyte migration regulation of protein kinase B signaling positive regulation of protein kinase B signaling cardiac cell development cardiac muscle cell development cardiac muscle contraction cellular response to methionine positive regulation of SCF-dependent proteasomal ubiquitin-dependent catabolic process negative regulation of calcineurin-NFAT signaling cascade cellular response to amino acid stimulus cellular response to leucine cellular response to hypoxia cellular response to osmotic stress regulation of cellular response to stress regulation of membrane permeability vascular endothelial cell response to laminar fluid shear stress antibacterial innate immune response regulation of cellular response to heat negative regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter positive regulation of wound healing, spreading of epidermal cells negative regulation of TORC2 signaling regulation of locomotor rhythm negative regulation of TORC1 signaling positive regulation of TORC1 signaling positive regulation of cytoplasmic translational initiation regulation of lysosome organization negative regulation of lysosome organization positive regulation of pentose-phosphate shunt cellular response to leucine starvation negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of cytoplasmic translation regulation of autophagosome assembly double-strand break repair via homologous recombination positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation regulation of glycogen biosynthetic process regulation of carbohydrate metabolic process protein phosphorylation response to stress positive regulation of gene expression cell growth phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation regulation of fatty acid beta-oxidation regulation of response to food regulation of GTPase activity regulation of carbohydrate utilization regulation of protein kinase activity protein autophosphorylation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of NFAT protein import into nucleus
          It has to be done as per old AB suggested Products section.

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