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Invitrogen
PDCD6IP Monoclonal Antibody (14D10)
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FIGURE: 1 / 9
PDCD6IP Antibody (MA5-36229) in ICC/IF
Immunocytochemistry analysis of PDCD6IP in U20S cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in PDCD6IP monoclonal antibody (Product # MA5-36229) at a dilution of 2 µg/mL (overnight, 4°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C).
Product Details
MA5-36229
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
14D10
Immunogen
E.coli-derived human PDCD6IP recombinant protein (Position: A2-D330).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human HepG2 whole cell, human Jurkat whole cell, human PANC-1 whole cell, human K562 whole cell, human SW579 whole cell, rat RH35 whole cell, mouse NIH3T3 whole cell. IHC: human mammary cancer tissue, human intestinal cancer tissue, human intestinal cancer tissue, mouse testis tissue, rat testis tissue, rat testis tissue. ICC/IF: U20S cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Apoptotic linked-gene-product 2 (ALG-2) interacting protein X (ALIX) is a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALG-2 interacting protein 1; ALG-2 interacting protein X; ALG-2-interacting protein 1; ALG-2-interacting protein X; Alg2-interacting protein 1; Alg2-interacting protein X; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; E2F1-inducible protein; Eig2; Hp95; MGC17003; PDCD6-interacting protein; Programmed cell death 6-interacting protein; similar to XP95; possible role in signal transduction and cell growth/death; unnamed protein product
Gene Aliases: AI480591; AIP1; ALIX; AW544830; C76364; DRIP4; Eig2; HP95; KIAA1375; MCPH29; mKIAA1375; PDCD6IP; RGD1561176
UniProt ID: (Human) Q8WUM4, (Rat) Q9QZA2, (Mouse) Q9WU78
Entrez Gene ID: (Human) 10015, (Rat) 501083, (Mouse) 18571
mitotic cytokinesis
actomyosin contractile ring assembly
apoptotic process
nucleus organization
mitotic metaphase plate congression
regulation of centrosome duplication
protein transport
macroautophagy
multivesicular body assembly
viral budding via host ESCRT complex
maintenance of epithelial cell apical/basal polarity
viral budding
protein homooligomerization
cell division
midbody abscission
bicellular tight junction assembly
regulation of membrane permeability
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
extracellular exosome biogenesis
regulation of mitotic spindle assembly
positive regulation of exosomal secretion
regulation of extracellular exosome assembly
positive regulation of extracellular exosome assembly
cell separation after cytokinesis
transport
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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